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MABS2158-25UG Anti-PECAM-1 (CD31) Antibody, clone C1096

Anti-PECAM-1 (CD31), clone C1096, Cat. No. MABS2158, is a mouse monoclonal antibody that detects Platelet endothelial cell adhesion molecule 1 (PECAM-1) and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MABS2158-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2158-25UG
DescriptionAnti-PECAM-1 (CD31) Antibody, clone C1096
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • EndoCAM
  • GPIIA
  • PECA1
  • CD31
Background InformationPlatelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA', PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PECAM-1 (CD31), clone C1096, Cat. No. MABS2158, is a mouse monoclonal antibody that detects Platelet endothelial cell adhesion molecule 1 (PECAM-1) and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PECAM-1 (CD31) in human kidney tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected PECAM-1 (CD31) in Jurkat cells.

Immunofluorescence Analysis: A representative lot detected PECAM-1 (CD31) in Immunofluorescence applications (Moerkamp, A.T., et. al. (2017). Stem Cells Dev. 26(21):1552-1565).

Immunoprecipitation Analysis: A representative lot immunoprecipitated PECAM-1 (CD31) in Immunoprecipitation applications (Moerkamp, A.T., et. al. (2017). Stem Cells Dev. 26(21):1552-1565).

Immunocytochemistry Analysis: A representative lot detected PECAM-1 (CD31) in Immunocytochemistry applications (Moerkamp, A.T., et. al. (2017). Stem Cells Dev. 26(21):1552-1565).

Western Blotting Analysis: A representative lot detected PECAM-1 (CD31) in Western Blotting applications (Moerkamp, A.T., et. al. (2017). Stem Cells Dev. 26(21):1552-1565).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected PECAM-1 (CD31) in Immunohistochemistry applications (Moerkamp, A.T., et. al. (2017). Stem Cells Dev. 26(21):1552-1565).
Biological Information
ImmunogenSca-1+ treated Human cardiac progenitor cells (CPCs).
CloneC1096
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone C1096 is a mouse monoclonal antibody that specifically detects human PECAM-1.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
Purification MethodProtein G purified
UniProt Number
Molecular Weight82.52 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected PECAM-1 (CD31) in human tonsil tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2158-25UG 04054839010477