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PC21 Anti-PDGF (Ab-1) (101-116) Rabbit pAb

This Anti-PDGF (Ab-1) (101-116) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections, ELISA for the detection of PDGF (Ab-1) (101-116).

Anti-PDGF (Ab-1) (101-116) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Platelet Derived Growth Factor

PC21
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody
Description
OverviewRecognizes the B subunit of PDGF in mesenchymal cells and fetal epithelial tissue.
Catalogue NumberPC21
Brand Family Calbiochem®
SynonymsAnti-Platelet Derived Growth Factor
References
ReferencesLaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
Ross, R., et al. 1986 Cell 46, 155.
Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlPC12 cells
Positive controlMesenchymal cells or fetal epithelial tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
ELISA (see comments)
Application CommentsThis antibody specific for the B form of human PDGF that will react by immunofluorescence with human platelets and has been reported to react in ELISA with the PDGF(AB) and PDGF(BB) protein. Antibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogena synthetic peptide (TEVFEISRRLIDRTNA) corresponding amino acids 101-116 of human PDGF B chain
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC21 0

Documentation

Anti-PDGF (Ab-1) (101-116) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-PDGF (Ab-1) (101-116) Rabbit pAb Certificates of Analysis

TitleLot Number
PC21

References

Reference overview
LaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
Ross, R., et al. 1986 Cell 46, 155.
Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-Platelet Derived Growth Factor
ApplicationImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
ELISA (see comments)
DescriptionPurified rabbit polyclonal antibody. Recognizes the B form of human PDGF.
BackgroundPlatelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The protein may exist as either a heterodimer AB or as a homodimer AA or BB. PDGF (BB) has been identified as the human homolog of the v-sis oncogene product and as such has been designated c-sis. The v-sis transforming domain is 89 amino acids in length and is identical in sequence to PDGF (BB). Binding of the PDGF molecule to its specific receptor occurs via interaction with a specific domain on the surface of the molecule known as the V4 surface domain. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (TEVFEISRRLIDRTNA) corresponding amino acids 101-116 of human PDGF B chain
IsotypeIgG
Specieshuman, rat
Positive controlMesenchymal cells or fetal epithelial tissue
Negative controlPC12 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody specific for the B form of human PDGF that will react by immunofluorescence with human platelets and has been reported to react in ELISA with the PDGF(AB) and PDGF(BB) protein. Antibody should be titrated for optimal results in individual sample types.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesLaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
Ross, R., et al. 1986 Cell 46, 155.
Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.