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MABE1017 Anti-OASIS/CREB3L1 Antibody, clone 44C7

MABE1017
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1017
DescriptionAnti-OASIS/CREB3L1 Antibody, clone 44C7
Alternate Names
  • Cyclic AMP-responsive element-binding protein 3-like protein 1
  • cAMP-responsive element-binding protein 3-like protein 1
  • Old astrocyte specifically-induced substance
  • OASIS
  • Processed cyclic AMP-responsive element-binding protein 3-like protein 1
  • OASIS/CREB3L1
Background InformationCyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt Q9Z125; also known as BBF-2 homolog, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance) is encoded by the Creb3l1 (also known as Oasis) gene (Gene ID 26427) in murine species. Creb3l1 belongs to the the old astrocyte specifically induced substance (OASIS) family of transcription factors that function as ER stress transducers. OASIS members possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain, as well as a transmembrane domain that allows them to associate with the ER. OASIS family members, except Luman (Creb3), exhibit cell- and tissue-specific expression patterns, with Oasis (Creb3l1) being preferentially expressed in osteoblasts and astrocytes, AIbZIP (Creb3l4, Creb4, Tisp40) in testis and prostate, Bbf2h7 (Creb3l2) in chondrocytes, and Crebh (Creb3l3) in hepatocytes. OASIS family members are activated in response to ER stress via a distinct mechanism than the ER unfold protein response (UPR) transducer ATF6. Oasis (Creb3l1) and Bbf2h7 (Creb3l2) are constantly ubiquitinated by the ER-resident E3 ubiquitin ligase HMG-CoA reductase degradation 1 (Hrd1) and degraded by proteasome under normal conditions. ER stress stabilizes Oasis and Bbf2h7 by inducing a dissociation of these transcription factors from Hrd1.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-OASIS/CREB3L1 Antibody, clone 44C7 is validated for use in Western Blotting for the detection of OASIS/CREB3L1.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected full-length OASIS in lysates from untreated MC3T3-E1 murine osteoblastic cells and cleaved N-terminal OASIS fragment in lysates from MC3T3-E1 cells subjected to ER stresser thapsigargin or brefeldin A treatment (Murakami, T., et al. (2009). Nat. Cell Biol. 11(10):1205-1211).
Western Blotting Analysis: A representative lot detected a higher OASIS expression level in Hrd1-/- than in wild-type MEFs. A more elevated OASIS level was also detected in osteoblasts differentiated from Hrd1-/- than from wild-type MEFs following bone morphogenetic protein 2 (BMP2) treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Biological Information
ImmunogenFlag-tagged recombinant protein corresponding to the N-terminal half of mouse OASIS/CREB3L1.
EpitopeN-terminal half
Clone44C7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms as well as the N-terminal fragment of cleaved Oasis.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Creb3l1
  • Oasis
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~62 and 52 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MC3T3-E1 mouse osteoblastic cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected OASIS/CREB3L1 in 10 µg of MC3T3-E1 mouse osteoblastic cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1017 04055977297447

Documentation

Anti-OASIS/CREB3L1 Antibody, clone 44C7 SDS

Title

Safety Data Sheet (SDS) 

Anti-OASIS/CREB3L1 Antibody, clone 44C7 Certificates of Analysis

TitleLot Number
Anti-OASIS/CREB3L1, clone 44C7 - 3389941 3389941
Anti-OASIS/CREB3L1, clone 44C7 - 4025552 4025552
Anti-OASIS/CREB3L1, clone 44C7 -Q2618566 Q2618566
Anti-OASIS/CREB3L1, clone 44C7 Monoclonal Antibody 2971088

References

Reference overviewPub Med ID
Activation of OASIS family, ER stress transducers, is dependent on its stabilization.
Kondo, S; Hino, SI; Saito, A; Kanemoto, S; Kawasaki, N; Asada, R; Izumi, S; Iwamoto, H; Oki, M; Miyagi, H; Kaneko, M; Nomura, Y; Urano, F; Imaizumi, K
Cell death and differentiation  19  1939-49  2012

Show Abstract
22705851 22705851
Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation.
Murakami, T; Saito, A; Hino, S; Kondo, S; Kanemoto, S; Chihara, K; Sekiya, H; Tsumagari, K; Ochiai, K; Yoshinaga, K; Saitoh, M; Nishimura, R; Yoneda, T; Kou, I; Furuichi, T; Ikegawa, S; Ikawa, M; Okabe, M; Wanaka, A; Imaizumi, K
Nature cell biology  11  1205-11  2009

Show Abstract
19767743 19767743