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MABF2096-25UG Anti-Norovirus (MNV-1) Antibody, clone 4F9.4

Anti-Norovirus (MNV-1), clone 4F9.4 Antibody, Cat. No. MABF2096, is a mouse monoclonal antibody that detects Norovirus (MNV-1) and has been tested for use in ELISA, Neutralizing, and Western Blotting.

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MABF2096-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABF2096-25UG
DescriptionAnti-Norovirus (MNV-1) Antibody, clone 4F9.4
Alternate Names
  • MNV-1
  • Murine Norovirus
Background InformationNorovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187 kDa polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59 kDa capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22 kDa basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. This monoclonal antibody, clone 4F9.4, recognizes the P domain portion of the viral capsid protein and effectively neutralize MNV-1 and WU20 strains. It can also neutralize recombinant viruses V339I and D348E at higher doses. (Ref.: Kolawole, AO et al. (2014). J. Gen. Virol. 95(9); 1958-1968).
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgA in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-Norovirus (MNV-1), clone 4F9.4 Antibody, Cat. No. MABF2096, is a mouse monoclonal antibody that detects Norovirus (MNV-1) and has been tested for use in ELISA, Neutralizing, and Western Blotting.
Key Applications
  • ELISA
  • Neutralizing
  • Western Blotting
Application NotesNeutralizing Analysis: A representative lot neutralized murine norovirus (MNV-1) in Dulbecco s modified Eagle's medium (DMEM) (Courtesy of Abimbola Kolawole, Ph.D. and Christiane Wobus, Ph.D., University of Michigan Medical School, Ann Arbor, Michigan USA).

ELISA Analysis: A representative lot detected Norovirus (MNV-1) in ELISA applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

Neutralizing Analysis: A representative lot effectively neutralized Norovirus (MNV-1) in Neutralizing applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

Western Blotting Analysis: A representative lot detected Norovirus (MNV-1) in Western Blotting applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).
Biological Information
ImmunogenInfectious murine norovirus-1
Clone4F9.4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4F9.4 specifically targets capsid protein of murine norovirus.
IsotypeIgAκ
Species Reactivity
  • Virus
Species Reactivity NoteMurine Norovirus.
Antibody TypeMonoclonal Antibody
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Molecular Weight187.47 kDa calculated.
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgA by isotyping testing.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
MABF2096-25UG 04054839659911