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487923 Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
487923
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
487923-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes nitrotyrosine.
Catalogue Number487923
Brand Family Calbiochem®
Application Data
Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, 0.02% sodium azide, pH 7.2.
Positive controlMouse macrophages treated with peroxynitrite
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (2 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenperoxynitrite-treated KLH
CloneCC22.8C7.3
HostMouse
IsotypeIgG2b
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
487923-50UG 04055977200379

Documentation

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) SDS

Title

Safety Data Sheet (SDS) 

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) Certificates of Analysis

TitleLot Number
487923

References

Reference overview
Myatt, L., et al. 1996. Hypertension 28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.

Citations

Title
  • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-February-2020 JSW
    ApplicationImmunoblotting (2 µg/ml)
    Application Data
    Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
    BackgroundNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by Nitric oxide synthase. The reaction of NO with superoxide which is often present under conditions of oxidative stress, forms peroxynitrite, a strong oxidant which can nitrosate tyrosine residues on proteins. Nitrotyrosine has been shown to be present in proteins from artherosclerotic lesions of human carotid arteries and also in placenta during pre-eclampsia.
    HostMouse
    Immunogenperoxynitrite-treated KLH
    CloneCC22.8C7.3
    IsotypeIgG2b
    Speciesa broad range of species
    Positive controlMouse macrophages treated with peroxynitrite
    FormLiquid
    FormulationIn PBS, 50% glycerol, 0.02% sodium azide, pH 7.2.
    Preservative≤0.1% sodium azide
    CommentsVariables associated with assay conditions will dictate the proper working dilution.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
    Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
    Citation
  • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.
    		•