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MABN2292-100UG Anti-Neuregulin-1 Antibody, clone 4F10

Anti-Neuregulin-1 clone 4F10, Cat. No. MABN2292, is a highly specific rat monoclonal antibody that targets Typ III NRG1 beta-1 and has been tested for use in Western Blotting.

Anti-Neuregulin-1 Antibody, clone 4F10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NRG1, GGF, HGL, HRGA, NDF, SMDF UniProt Number: Q02297-6
MABN2292-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2292-100UG
DescriptionAnti-Neuregulin-1 Antibody, clone 4F10
Alternate Names
  • NRG1
  • Acetylcholine receptor-inducing activity
  • ARIA
  • Breast cancer cell differentiation factor p45
  • Glial growth factor
  • Heregulin
  • HRG
  • Neu differentiation factor
  • Sensory and motor neuron-derived factor
Background InformationNeuregulin-1 (UniProt: Q02297; also known as Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, HRG, Neu differentiation factor, Sensory and motor neuron-derived factor) is encoded by the NRG1 (also known as GGF, HGL, HRGA, NDF, SMDF) gene (Gene ID: 3084) in human. Neuregulin-1 is a single-pass type 1 membrane protein that acts as a direct ligand for ErbBB3 and ErbB4 tyrosine kinase receptors. It concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Eleven isoforms of Neuregulin-1 are known that are produced by alternative splicing. They have been classified as type I (isoforms with an Ig domain and a glycosylation domain) type II NRGs (isoforms with an Ig domain, but no glycosylation domain). These multiple isoforms perform diverse cellular functions, such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells and inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction. Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. Dual cleavage of Neuregulin-1 by beta-secretase 1 (BACE1) and ADAM17 is shown to liberate EGF-like domain, which stimulates ErbB3 signaling. (Ref.: Fleck, D., et a. (2013). J. Neurosci. 33(18):7856-7869).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Neuregulin-1 clone 4F10, Cat. No. MABN2292, is a highly specific rat monoclonal antibody that targets Typ III NRG1 beta-1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Neuregulin-1 in Western Blotting applications (Fleck, D., et. al. (2013). J Neurosci. 33(18):7856-69; Cheret, C., et. al. (2013). EMBO J. 32(14):2015-28; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33; Ben Halima, S., et. al. (2016). Cell Rep. 14(9):2127-2141).
Biological Information
ImmunogenA linear peptide corresponding to 10 amino acids from the N-terminal, extracellular domain of human Neuregulin 1, isoform 6.
Epitopeextracellular domain
Clone4F10
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 4F10 is a rat monoclonal antibody that detects human Neuregulin-1. It targets an epitope within 10 amino acids from the N-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Chimpanzee based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NRG1
  • GGF
  • HGL
  • HRGA
  • NDF
  • SMDF
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 71 kDa observed; 71.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with Type I NRG1 and Type III NRG1 and treated with BACE inhibitor.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neuregulin-1 in HEK293 cells transfected with Type I NRG1 and Type III NRG1 and treated with BACE inhibitor.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2292-100UG 04054839482267