Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB5731-25UG Anti-Nanog, NT

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.

AB5731-25UG
25 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed
Description
Catalogue NumberAB5731-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nanog, NT
Alternate Names
  • Homeobox transcription factor Nanog
  • Nanog homeobox
Background InformationNANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).
References
Product Information
FormatAffinity Purified
Control
  • Whole cell lysate from mouse embryonic stem cells.
PresentationPurified rabbit polyclonal immunoglobulin in PBS buffer containing liquid 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide from mouse Nanog.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityAntibody recognizes Nanog.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLJ12581
  • FLJ40451
  • hNanog
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFunction: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation By similarity.

Subunit structure: Interacts with SMAD1 and SALL4 By similarity.

Subcellular location: Nucleus. Ref.10

Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.5 Ref.8

Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.10 Ref.3 Ref.8 Ref.6 Ref.9

Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.

Sequence similarities Belongs to the Nanog homeobox family.

Contains 1 homeobox DNA-binding domain.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight39 kDa (MW 34 kDa)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5731-25UG 04054839342684

Related Products & Applications

Product Families

Small Pack Size Antibodies

Find smaller, more convenient sizes of our highly characterized, extensively published and high-quality antibodies.
Learn More >>