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DR1089 Anti-NKX2-5 Mouse mAb (1E4-G5)

This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.

Anti-NKX2-5 Mouse mAb (1E4-G5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NK2 Transcription Factor Related 5

DR1089
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.
Catalogue NumberDR1089
Brand Family Calbiochem®
SynonymsAnti-NK2 Transcription Factor Related 5
Application Data
Detection of human NKX2-5 by immunoblotting. Sample: Whole tissue extract from human thyroid. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NKX2-5 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.
References
ReferencesYadava, R,S., et al. 2008. Nat. Genet. 40 61.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHuman thyroid tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
Clone1E4-G5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1089 0

Documentation

Anti-NKX2-5 Mouse mAb (1E4-G5) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Yadava, R,S., et al. 2008. Nat. Genet. 40 61.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-NK2 Transcription Factor Related 5
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human NKX2-5 by immunoblotting. Sample: Whole tissue extract from human thyroid. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NKX2-5 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.
BackgroundNKX2-5 is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases
HostMouse
ImmunogenFull-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
Clone1E4-G5
IsotypeIgG₁
Specieshuman
Positive controlHuman thyroid tissue, HeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesYadava, R,S., et al. 2008. Nat. Genet. 40 61.