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CA1029 Anti-N-Cadherin Mouse mAb (13A9)

CA1029
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~140 kDa N-cadherin protein in HeLa cells.
Catalogue NumberCA1029
Brand Family Calbiochem®
Application Data
Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
References
ReferencesChristofori, G. 2006. Nature 44, 444.
Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
Product Information
FormLiquid
FormulationUndiluted ascites.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1:100)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
ImmunogenHuman
Clone13A9
HostMouse
IsotypeIgG
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
CA1029 0