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MABN2620-25UG Anti-Myelin Proteolipid Protein Antibody, clone AA3

Anti-Myelin Proteolipid Protein, clone AA3, Cat. No. MABN2620, is a rat monoclonal antibody that detects Myelin proteolipid protein and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blot.

MABN2620-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2620-25UG
DescriptionAnti-Myelin Proteolipid Protein Antibody, clone AA3
Alternate Names
  • PLP
  • Lipophilin
Background InformationMyelin proteolipid protein (UniProt: P04116; also known as PLP, DM20, Lipophilin) is encoded by the PLP1 (also known as PLP) gene (Gene ID: 281410) in bovine. Myelin proteolipid protein is a highly conserved, multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is composed of a series of alternating hydrophobic and hydrophilic domains. It is the major myelin protein from the central nervous system and plays an important role in the formation or maintenance of the multilamellar structure of myelin. It is shown to be the most abundant protein of the central nervous system (CNS) myelin sheath with a relative abundance of about 17% of the myelin proteins. It is mainly expressed by myelin forming oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system. PLP1 gene is reported to encode two isoforms; a 30 kDa PLP and a 25 kDa DM20 that has a deletion of 35 amino acids in the second cytoplasmic domain. The DM20 protein is expressed prior to PLP during development and as development proceeds PLP becomes the more predominant protein. Although the molecular mass of PLP is ~ 30 kDa, it migrates anomalously on SDS gels and gives a lower apparent molecular mass. (Ref.: LeVine, SM., et al. (1990). Dev. Neurosci. 12(4-5); 235-250; Yamamura, T., et al. (1991). J. Neurochem. 57(5); 1671-1680; Sarret, C., et al. (2010). Neuroscience. 166(2); 522-538).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Myelin Proteolipid Protein, clone AA3, Cat. No. MABN2620, is a rat monoclonal antibody that detects Myelin proteolipid protein and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blot.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myelin Proteolipid Protein in HepG2 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Myelin Proteolipid Protein in human cerebral cortex and human cerebellum tissue sections.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Myelin Proteolipid Protein in Immunoprecipitation applications (Gudz, T.I., et. al. (2006). J Neurosci. 26(9):2458-66).

ELISA Analysis: A representative lot detected Myelin Proteolipid Protein in ELISA applications (Yamamura, T., et. al. (1991). J Neurochem. 57(5):1671-80).

Immunohistochemistry Applications: A representative lot detected Myelin Proteolipid Protein in Immunohistochemistry applications ( Gudz, T.I., et. al. (2006). J Neurosci. 26(9):2458-66; Sarret, C., et. al. (2010). Neuroscience. 166(2):522-38).

Western Blotting Analysis: A representative lot detected Myelin Proteolipid Protein in Western Blotting applications (Zhu, H., et. al. (2012). Glia. 60(1):69-82).

Immunocytochemistry Analysis: A representative lot detected Myelin Proteolipid Protein in Immunocytochemistry applications (Sarret, C., et. al. (2010). Neuroscience. 166(2):522-38).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified Myelin proteolipid protein from bovine brain.
EpitopeC-terminal
CloneAA3
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone AA3 is a rat monoclonal antibody that detects Myelin proteolipid protein. It targets an epitope within 13 amino acids from the C-terminal region. It detects both isoforms of Myelin proteolipid protein.
IsotypeIgG2bκ
Species Reactivity
  • Rat
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PLP1
  • PLP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~17 kDa observed; 30.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myelin Proteolipid Protein in SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2620-25UG 04065266020755

Documentation

Anti-Myelin Proteolipid Protein Antibody, clone AA3 SDS

Title

Safety Data Sheet (SDS) 

Anti-Myelin Proteolipid Protein Antibody, clone AA3 Certificates of Analysis

TitleLot Number
Anti-Myelin Proteolipid Protein, clone AA3 - 4023763 4023763
Anti-Myelin Proteolipid Protein, clone AA3 - 4025561 4025561
Anti-Myelin Proteolipid Protein, clone AA3 - 4028623 4028623
Anti-Myelin Proteolipid Protein, clone AA3 - 4033104 4033104
Anti-Myelin Proteolipid Protein, clone AA3 - 4034325 4034325
Anti-Myelin Proteolipid Protein, clone AA3 - 4038978 4038978
Anti-Myelin Proteolipid Protein, clone AA3 - 4042599 4042599
Anti-Myelin Proteolipid Protein, clone AA3 - 4044764 4044764
Anti-Myelin Proteolipid Protein, clone AA3 - 4074328 4074328
Anti-Myelin Proteolipid Protein, clone AA3 - 4137663 4137663
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