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MABF2818-25UG Anti-Medullary epithelium Antibody, clone ER-TR5

Anti-Medullary epithelium, clone ER-TR5, Cat. No. MABF2818, is a rat monoclonal antibody that detects Medullary epithelium and is tested for use in Immunohistochemistry and Immunofluorescence.

MABF2818-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2818-25UG
DescriptionAnti-Medullary epithelium Antibody, clone ER-TR5
Alternate Names
  • mTEC
Background InformationThe thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTEC) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. They participate in self-tolerance by eliminating self-reactive T cells. In murine species, mTEC are further divided into mTEC low and mTEC high according to the expression levels of several maturation molecules, such as MHCII and CD8o. mTEC low cells can serve as precursors for mature mTEC high cells. mTECs have also been classified into four major subsets, termed mTEC I-IV depending on their distinct transcriptional and molecular characteristics. mTEC I and II subpopulation appear early and can be detected at E18.5 and are most proliferative cells. mTEC III are detected in thymus at 4-weeks. mTEC IV are visible on day 6 in neonatal mice. The function of mTECs is dependent on their characteristic features such as ectopic expression of peripheral tissue restricted antigens and their master regulator-autoimmune regulator (Aire), expression of various chemokines and cytokines. Lymphotoxin β receptor (LTβR) is reported to be an essential regulator of multiple mTEC subsets within the mTEC low compartment. It controls thymic tolerance by regulating the frequency and makeup of intrathymic dendritic cells that are required for effective thymocyte negative selection. Clone ER-TR5 is shown to exclusively react with mTECs. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Lucas, B., et al. (2020). Nat. Commun. 11; Article 2198; Cosway, EJ., et al. (2017). J. Exp. Med. 214(11); 3183-3195; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Medullary epithelium, clone ER-TR5, Cat. No. MABF2818, is a rat monoclonal antibody that detects Medullary epithelium and is tested for use in Immunohistochemistry and Immunofluorescence.
Key Applications
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested applications

Immunofluorescence Analysis: A representative lot detected Medullary epithelium in Immunofluorescence applications (Akiyama, T., et al. (2005). Science. 308(5719):248-51; Lei, Y., et al. (2011). J Exp Med. 208(2):383-94; Cosway, E.J., et al. (2017). J Exp Med. 214(11):3183-3195).

Immunohistochemistry Applications: A representative lot detected Medullary epithelium in Immunohistochemistry applications (Van Vliet, E., et al. (1984). Eur J Immunol. 14(6):524-9; Akiyama, T., et al. (2005). Science.;308(5719):248-51; Dooley, J., et al. (2006). J Immunol. 176(11):6484-90; Lei, Y., et al. (2011). J Exp Med. 208(2):383-94; Cosway, E.J., et al. (2017). J Exp Med. 214(11):3183-3195).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenThymic stromal cells isolated from C3H mice.
EpitopeUnknown
CloneER-TR5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone ER-TR5 is a rat monoclonal antibody that detects Medullary epithelial cells.
IsotypeIgM
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodLigatrap
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in Mouse thymus tissue.

Immunohistochemistry Applications: 1:150 dilution of this antibody detected Medullary epithelium in Mouse thymus tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2818-25UG 04065268900789