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IM1014 Anti-Matriptase/MT-SP1 Rabbit pAb

This Anti-Matriptase/MT-SP1 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Matriptase/MT-SP1.

Anti-Matriptase/MT-SP1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14

IM1014
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
IM1014-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~75 kDa matriptase protein in MCF-7 cells.
Catalogue NumberIM1014
Brand Family Calbiochem®
SynonymsAnti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14
Application Data
Lane 1: Detection of human Matriptase/MT-SP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF-7 cells. Primary antibody: Anti-Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (1:1000). Detection: chemiluminescence. Lane 2,3: Detection of human Matripatase/MT-SP1 by immunoprecipitation (IP) followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF-7 cells. Antibody for IP (Lane 2): Anti- Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (20 μg/mg total protein). Negative control (Lane 3): Purified rabbit IgG (20 μg/mg total protein). Immunoblotting conditions: same as Lane 1.
References
ReferencesSantin, A.D., et al. 2003. Cancer 98, 1898.
Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlMCF-7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:500-1:2000)
Immunoprecipitation (5-20 µg/mg lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human matriptase
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM1014-50UG 04055977228175

Documentation

Anti-Matriptase/MT-SP1 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Matriptase/MT-SP1 Rabbit pAb Certificates of Analysis

TitleLot Number
IM1014

References

Reference overview
Santin, A.D., et al. 2003. Cancer 98, 1898.
Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14
ApplicationImmunoblotting (1:500-1:2000)
Immunoprecipitation (5-20 µg/mg lysate)
Application Data
Lane 1: Detection of human Matriptase/MT-SP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF-7 cells. Primary antibody: Anti-Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (1:1000). Detection: chemiluminescence. Lane 2,3: Detection of human Matripatase/MT-SP1 by immunoprecipitation (IP) followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF-7 cells. Antibody for IP (Lane 2): Anti- Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (20 μg/mg total protein). Negative control (Lane 3): Purified rabbit IgG (20 μg/mg total protein). Immunoblotting conditions: same as Lane 1.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~75 kDa matriptase protein.
BackgroundMatriptase (MT-SP1, ACC. No. NP_068813), a type II membrane serine protease, may play important roles in cell migration and tumor cell metastasis. It is a mosaic protein with broad spectrum cleavage activity and two potential regulatory modules. Matriptase can convert hepatocyte growth factor/scatter factor to its active form, which can then activate c-Met tyrosine phosphorylation. Furthermore, protease-activated receptor 2 (PAR2) and enzymatically inactive single-chain uPA (pro-uPA) were identified as substrates of matriptase. Matriptase may regulate PAR2 mediated functions such as inflammatory responses, cell adhesion, and uPA-dependent tumor cell invasion and metastasis. The expression of matriptase is associated with a more invasive phenotype.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human matriptase
IsotypeIgG
Specieshuman
Positive controlMCF-7 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Highly Toxic
ReferencesSantin, A.D., et al. 2003. Cancer 98, 1898.
Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.