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MABT1549-25UG Anti-MT5-MMP/MMP24 Antibody, clone 3D2

Anti-MT5-MMP/MMP24, clone 3D2, Cat. No. MABT1549, is a rat monoclonal antibody that detects MT5-MMP and is tested for use in Western Blotting.

MABT1549-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1549-25UG
DescriptionAnti-MT5-MMP/MMP24 Antibody, clone 3D2
Alternate Names
  • Matrix metalloproteinase-24
  • MMP-24
  • Matrix metalloproteinase-21
  • MMP-21
  • Membrane-type matrix metalloproteinase 5
  • MTMMP5
  • Membrane-type-5 matrix metalloproteinase
  • MT5MMP
Background InformationMatrix metalloproteinase-24 (UniProt: Q9R0S2; also known as MMP-24, Matrix metalloproteinase-21, MMP-21, Membrane-type matrix metalloproteinase 5, MT-MMP 5, MTMMP5, Membrane-type-5 matrix metalloproteinase, MT5-MMP, MT5MMP) is encoded by the Mmp24 (also known as Mmp21, Mt5mmp) gene (Gene ID: 17391) in murine species. MMP-24 is a single-pass type I membrane protein that is mainly expressed in neuronal cells of both central and peripheral nervous systems. It mediates the cleavage of N-cadherin and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. It is also involved in cell-cell interactions between nociceptive neurites and mast cells. MMP-24 is synthesized with a signal peptide (aa 1-41) and a propeptide (aa 42-128) that are subsequently cleaved off to generate functional MMP-24 (aa 129-618) or processed MMP-24 (aa 129-554). MMP-24 contains a cysteine switch motif (aa 110-117) that binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release results in the activation of enzyme.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MT5-MMP/MMP24, clone 3D2, Cat. No. MABT1549, is a rat monoclonal antibody that detects MT5-MMP and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human MT5-MMP.
EpitopeExtracellular domain
Clone3D2 also termed M24N 3D2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 3D2 is a rat monoclonal antibody that detects MT5-MMP/MMP-24. It targets an epitope within 16 amino acids from the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Mmp24
  • Mmp21
  • Mt5mmp
Purification MethodProtein G purified
UniProt Number
Molecular Weight70.46 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1549-25UG 04065265651424