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IM61 Anti-MMP-9 (Ab-7) Mouse mAb (GE-213)

Anti-MMP-9 (Ab-7) Mouse mAb (GE-213) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Type IV Collagenase, Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9

IM61
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~92 kDa latent and the ~78 kDa active forms of MMP-9 in dexamethasone-treated HT1080 cells, HUVEC, and breast carcinoma tissue. Also recognizes the ~200 kDa MMP-9/TIMP-1 complex. Does not cross-react with MMP-2.
Catalogue NumberIM61
Brand Family Calbiochem®
SynonymsAnti-Type IV Collagenase, Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9
Application Data
Detection of human MMP-9 by immunoprecipitation. Sample: Human MMP-9. Primary antibody: Anti-MMP-9 (Ab-7) Mouse mAb (GE-213) (Cat. No. IM61) (assay dependent). Detection: chemiluminescence.
References
ReferencesNikkari, S.T., et al. 1996. Amer. J. Pathol. 149, 1427.
Visscher, D.W., et al. 1994. Intl. J. Cancer 59, 339.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Product Information
DeclarationNot available for sale in Japan.
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlConditioned serum-free medium from dexamethasone-treated HT-1080 or HUVEC cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379. Soini, Y., et al. 1994. J. Histochem. Cytochem. 42, 945.
Key Applications Frozen Sections
Immunoprecipitation
Application NotesFrozen Sections (1:100)
Immunoprecipitation (10 µl/mg protein lysate)
Application CommentsPositive controls include cytotrophoblastic columns, endothelial and fibroblastic stromal cells of villi, and the large decidual membrane in placenta, macrophages in arteries and fibroblast, endothelial and tumor cells in breast carcinomas as well as conditioned serum free medium from dexamethasone-treated HT1080 or HUVEC cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, human MMP-9
ImmunogenHuman
CloneGE-213
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM61 0

Documentation

Anti-MMP-9 (Ab-7) Mouse mAb (GE-213) Certificates of Analysis

TitleLot Number
IM61

References

Reference overview
Nikkari, S.T., et al. 1996. Amer. J. Pathol. 149, 1427.
Visscher, D.W., et al. 1994. Intl. J. Cancer 59, 339.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-April-2009 RFH
SynonymsAnti-Type IV Collagenase, Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9
ApplicationFrozen Sections (1:100)
Immunoprecipitation (10 µl/mg protein lysate)
Application Data
Detection of human MMP-9 by immunoprecipitation. Sample: Human MMP-9. Primary antibody: Anti-MMP-9 (Ab-7) Mouse mAb (GE-213) (Cat. No. IM61) (assay dependent). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with purified the specified immunogen and fusing the splenocytes with mouse myeloma p3-X63-Ag8.653 cells (see application references). Recognizes the ~92 kDa latent and the ~78 kDa active forms of MMP-9 and the ~200 kDa MMP-9/TIMP-1 complex.
BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). MMP-9 also known as gelatinase B, 92 kDa gelatinase/type IV collagenase (EC 3.4.24.35) has a broad range of substrate specificity for native collagens including types IV, V, VII and X as well as gelatin, proteoglycans and elastin. MMP-9 is secreted as a 92 kDa proenzyme and can be activated in vitro by organomercurials (e.g., 4-aminophenylmercuric acetate, APMA), trypsin, and α-chymotrypsin and in vivo by cathepsin G and MMP-3 to the 83 kDa active form. The activity of MMP-9 can be inhibited by TIMPs in a 1:1 molar ratio. TIMP-1 is known to bind exclusively to pro-MMP-9 (Kd approximately 35 nM) whereas TIMP-2 binds to pro-MMP-2 with a Kd approximately 5 nM. MMP-9 has been measured in human plasma from normal individuals and has been shown to be elevated in sera of patients with hepatocellular carcinomas.
HostMouse
Immunogen speciesHuman
Immunogenpurified, human MMP-9
CloneGE-213
IsotypeIgG₁
Specieshuman
Positive controlConditioned serum-free medium from dexamethasone-treated HT-1080 or HUVEC cells or breast carcinoma tissue
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsPositive controls include cytotrophoblastic columns, endothelial and fibroblastic stromal cells of villi, and the large decidual membrane in placenta, macrophages in arteries and fibroblast, endothelial and tumor cells in breast carcinomas as well as conditioned serum free medium from dexamethasone-treated HT1080 or HUVEC cells. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesNikkari, S.T., et al. 1996. Amer. J. Pathol. 149, 1427.
Visscher, D.W., et al. 1994. Intl. J. Cancer 59, 339.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Application referencesOriginal Clone Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379. Soini, Y., et al. 1994. J. Histochem. Cytochem. 42, 945.