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IM38 Anti-MMP-8 (Ab-1) Mouse mAb (115-13D2)

Anti-MMP-8 (Ab-1) Mouse mAb (115-13D2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Matrix Metalloproteinase 8, Anti-Neutrophil Collagenase

IM38
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~85 kDa latent and ~64 kDa active forms of MMP-8.
Catalogue NumberIM38
Brand Family Calbiochem®
SynonymsAnti-Matrix Metalloproteinase 8, Anti-Neutrophil Collagenase
Application Data
Detection of human MMP-8 by immunoblotting. Sample: Tissue extract from human placenta. Primary antibody: Anti-MMP-8 (Ab-1) Mouse mAb (115-13D2) (Cat. No. IM38) (5 µg/ml). Detection: chemiluminescence.
References
ReferencesTakino, T., et al. 1995. J. Biol. Chem. 270, 23013.
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Knauper, V., et al. 1993. Biochem. J. 291, 847.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Blaser, J., et al. 1991. Eur. J. Biochem. 202, 1223.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Knauper, V., et al. 1990. Eur. J. Biochem. 189, 295.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1, 99.
Lazarus, G.S., et al. 1968. J. Clin. Invest. 47, 2622.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Preservative≤0.1% sodium azide
Applications
Application ReferencesParaffin Sections Matsuki, H., et al. 1996. Clin. Chim Acta 244, 129.
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (5 µg/ml)
Paraffin Sections (see application references)
Application CommentsThis antibody does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, or MMP-13. For paraffin sections, fix with PLP (perioddate-lysine-4% paraformaldehyde) and culture tissue in monensin prior to fixation (see application reference). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman MMP-8
ImmunogenHuman
Clone115-13D2
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM38 0

Documentation

Anti-MMP-8 (Ab-1) Mouse mAb (115-13D2) Certificates of Analysis

TitleLot Number
IM38

References

Reference overview
Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Knauper, V., et al. 1993. Biochem. J. 291, 847.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Blaser, J., et al. 1991. Eur. J. Biochem. 202, 1223.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Knauper, V., et al. 1990. Eur. J. Biochem. 189, 295.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1, 99.
Lazarus, G.S., et al. 1968. J. Clin. Invest. 47, 2622.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2007 JSW
SynonymsAnti-Matrix Metalloproteinase 8, Anti-Neutrophil Collagenase
ApplicationImmunoblotting (5 µg/ml)
Paraffin Sections (see application references)
Application Data
Detection of human MMP-8 by immunoblotting. Sample: Tissue extract from human placenta. Primary antibody: Anti-MMP-8 (Ab-1) Mouse mAb (115-13D2) (Cat. No. IM38) (5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~85 kDa latent and the ~64 kDa active forms of MMP-8.
BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the eleven proteins reported to date, ten are normally found as soluble molecules. MMP-8, also known as polymorphonuclear leukocyte collagenase, is a collagenolytic enzyme first described in 1986. MMP-8 is closely related to the fibroblast collagenases (47% homology) but is encoded by a different gene. The enzyme exists as an inactive pro form with a molecular weight of approximately 85 kDa of which 35% is contributed by glycosylation. MMP-8 becomes activated by removal of the amino terminal pro-peptide which can be effected by trypsin but not by cell bound plasmin in a two step process. MMP-8 also will undergo autoproteolysis a process which can also be mediated by mercurial compounds. Both processes are inhibited by TIMP-1 and TIMP-2, which bind to the catalytic domain of the enzyme with a 1:1 stoichiometry and is partially resistant to dissociation under SDS-PAGE conditions. The active enzyme has been found to be unstable at 37°C, breaking down into two fragments of 40 kDa and about 27 kDa. Of the two fragments the 40 kDa one has been identified as the catalytic domain and is biologically active while the other is the COOH terminal domain and is inactive.
HostMouse
Immunogen speciesHuman
Immunogenhuman MMP-8
Clone115-13D2
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, or MMP-13. For paraffin sections, fix with PLP (perioddate-lysine-4% paraformaldehyde) and culture tissue in monensin prior to fixation (see application reference). Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTakino, T., et al. 1995. J. Biol. Chem. 270, 23013.
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Knauper, V., et al. 1993. Biochem. J. 291, 847.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Blaser, J., et al. 1991. Eur. J. Biochem. 202, 1223.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Knauper, V., et al. 1990. Eur. J. Biochem. 189, 295.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1, 99.
Lazarus, G.S., et al. 1968. J. Clin. Invest. 47, 2622.
Application referencesParaffin Sections Matsuki, H., et al. 1996. Clin. Chim Acta 244, 129.