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MAB3317 Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7

MAB3317
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RELISA, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3317
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-14 Antibody, hemopexin domain, clone 113-5B7
Alternate Names
  • MT1-MMP
Product Information
FormatPurified
Control
  • Breast carcinoma tissue
PresentationPurified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.
Quality LevelMQ100
Applications
ApplicationAnti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blot: 10 μg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.


When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.


Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.


Immunoprecipitation


EIA


Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenCDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP
Epitopea.a. 319-333
Clone113-5B7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.
IsotypeIgG3κ
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Gene Symbol
  • MMP14
  • MTMMP1
  • MMP-14
  • MT1MMP
  • MT1-MMP
  • MMP-X1
  • EC 3.4.24.80
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Molecular Weight~59-69 kDa
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Manufactured by Daiichi Fine Chemical Co., Ltd
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB3317 04053252505065