Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF1413 Anti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody

This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor™ 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD74, Ii UniProt Number: P04441
MABF1413
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCRvioletFluor™ 450Monoclonal Antibody
Description
Catalogue NumberMABF1413
DescriptionAnti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody
Alternate Names
  • H-2 class II histocompatibility antigen gamma chain
  • Ia antigen-associated invariant chain (Ii)
  • MHC class II-associated invariant chain
  • CD74
  • MHC class II (I-A/I-E)
Background InformationThe M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
References
Product Information
FormatvioletFluor™ 450
PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor™ 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneM5/114.15.2
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD74
  • Ii
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1413 04055977291407

Related Products & Applications

Related Products

Catalogue Number Description
MABF1411 Anti-MHC class II (I-A/I-E), PE, clone M5/114 .15.2 Antibody
MABF1412 Anti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody
MABF1414 Anti-MHC class II (I-A/I-E), redFluor™ 710, clone M5/114 .15.2 Antibody
MABF1415 Anti-MHC class II (I-A/I-E), redFluor™ 710, clone M5/114 .15.2 Antibody
MABF33 Anti-MHC class II (I-A/I-E) Antibody, clone M5/114
MABF805 Anti-MHC class II (I-A/I-E), APC, clone M5/114 .15.2 Antibody
MABF806 Anti-MHC class II (I-A/I-E), APC, clone M5/114 .15.2 Antibody
MABF808 Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody
MABF809 Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody
MABF810 Anti-MHC class II (I-A/I-E), PE, clone M5/114 .15.2 Antibody

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies