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ST1642 Anti-MAP4K5 Mouse mAb (3H3)

Anti-MAP4K5 Mouse mAb (3H3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5

ST1642
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~100 kDa MAP4K5 protein.
Catalogue NumberST1642
Brand Family Calbiochem®
SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5
Application Data
Detection of human MAP4K5 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with MAP4K5 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MAP4K5 Mouse mAb (3H3) (Cat. No. ST1642) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 361-470 human MAP4K5, expressed as GST fusion protein
Clone3H3
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1642 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human MAP4K5 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with MAP4K5 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MAP4K5 Mouse mAb (3H3) (Cat. No. ST1642) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa MAP4K5 protein.
BackgroundThis gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 361-470 human MAP4K5, expressed as GST fusion protein
Clone3H3
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review