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AB3256-I-25UL Anti-Luciferase

Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-Luciferase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: N/A UniProt Number: P08659
AB3256-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3256-I-25UL
DescriptionAnti-Luciferase
Alternate Names
  • Luciferin 4-monooxygenase
Background InformationLuciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid.
Biological Information
ImmunogenGST-tagged full length recombinant firefly luciferase.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects firefly luciferase.
IsotypeIgG
Species Reactivity
  • All
Species Reactivity NoteAll
Antibody TypePolyclonal Antibody
Gene Symbol
  • N/A
Purification MethodAffinity Purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3256-I-25UL 04054839438035