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434631 Anti-Lewis x Mouse mAb (P12)

This Anti-Lewis x Mouse mAb (P12) is validated for use in Paraffin Sections for the detection of Lewis x.

Anti-Lewis x Mouse mAb (P12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Lex

434631
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the Lewis x carbohydrate chain in normal kidney.
Catalogue Number434631
Brand Family Calbiochem®
SynonymsAnti-Lex
References
ReferencesRee, H.J., et al. 1991. Cancer 67, 1338.
Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Product Information
FormLiquid
FormulationTissue Culture Supernatant
Positive controlNormal kidney tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Paraffin Sections
Application NotesParaffin Sections (1:10-1:120)
Application CommentsFor paraffin sections, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. Variables associated with assay conditions will dictate the proper working dilutions.
Biological Information
Immunogenfresh (non-cultured) human placenta cells
ImmunogenHuman
CloneP12
HostMouse
IsotypeIgM
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
434631 0

Documentation

Anti-Lewis x Mouse mAb (P12) SDS

Title

Safety Data Sheet (SDS) 

Anti-Lewis x Mouse mAb (P12) Certificates of Analysis

TitleLot Number
434631

References

Reference overview
Ree, H.J., et al. 1991. Cancer 67, 1338.
Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-June-2017 JSW
SynonymsAnti-Lex
ApplicationParaffin Sections (1:10-1:120)
DescriptionPurified mouse monoclonal antibody. Recognizes the Lewis x type 2 oligosaccharide chain.
BackgroundBlood group antigens are a group of molecules formed by sequential addition of saccharides to carbohydrate side chains of lipids and proteins and are detected on erythrocytes, certain epithelial cells and in secretions of some individuals. A, B and H antigens are synthesized by erythrocytes and integrated into their cell membranes. Lewis antigens are not synthesized by erythrocytes but can be absorbed from the plasma onto the surface of red blood cells. Subtle differences in the composition and arrangement of the sugars in the chains are sufficient to generate these distinct specificities. Lewis x is detected in most metastases and invasive cancer cells.
HostMouse
Immunogen speciesHuman
Immunogenfresh (non-cultured) human placenta cells
CloneP12
IsotypeIgM
Specieshuman
Positive controlNormal kidney tissue
FormLiquid
FormulationTissue Culture Supernatant
Preservative≤0.1% sodium azide
CommentsFor paraffin sections, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. Variables associated with assay conditions will dictate the proper working dilutions.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesRee, H.J., et al. 1991. Cancer 67, 1338.
Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.