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ABC2075-200UL Anti-LC3C

Anti-LC3C, Cat. No. ABC2075, is a rabbit polyclonal antibody that detects Microtubule-associated proteins 1A/1B light chain 3C and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-LC3C MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MAP1LC3C UniProt Number: Q9BXW4
ABC2075-200UL
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC2075-200UL
DescriptionAnti-LC3C
Alternate Names
  • Microtubule-associated proteins 1A/1B light chain 3C
  • Autophagy-related protein LC3 C
  • Autophagy-related ubiquitin-like modifier LC3C
  • MAP1 light chain 3-like protein 3
  • MAP1A/MAP1B light chain 3 C
  • MAP1A/MAP1B LC3 C
  • Microtubule-associated protein 1 light chain 3 gamma
Background InformationMicrotubule-associated proteins 1A/1B light chain 3C (UniProt: Q9BXW4; also known as Autophagy-related protein LC3C, Autophagy-related ubiquitin-like modifier LC3C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, MAP1A/MAP1B LC3 C, Microtubule-associated protein 1 light chain 3 gamma) is encoded by the MAP1LC3C gene (Gene ID: 440738) in human. LC3s (MAP1-LC3A, B and C) are structural proteins of autophagosomal membranes, widely used as biomarkers of autophagy. LC3A is mainly localized to perinuclear and nuclear regions, LC3B is evenly distributed throughout the cytoplasm and is also localized in the nucleolar regions, and LC3C is shown to be located in the cytoplasm and strongly in the nuclei. LC3C is synthesized with a propeptide (aa 127-147), which is removed in the mature form. LC3C is a ubiquitin-like modifier that plays a crucial role in antibacterial autophagy through the selective binding of CALCOCO2. It recruits all ATG8 family members to infecting bacteria such as S.Typhimurium. It is also reported to play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. LC3C is recruited to damaged mitochondria where it mediates autophagosomal degradation of mitochondrial parts in a piecemeal fashion and is required for maintaining basal mitochondrial network homeostasis. It also mediates autophagosomal degradation of MTX1 protein and cooperates with p62 to deliver MTX1 to lysosomes. (Ref.: Koukourakis, KI et al. (2015). PLoS One DOI:10.1371/journal.pone.0137675; Le Guerroue´ et al., 2017, Mol. Cell 68(4); 786-796).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-LC3C, Cat. No. ABC2075, is a rabbit polyclonal antibody that detects Microtubule-associated proteins 1A/1B light chain 3C and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected LC3C in rat brain tissue sections.
Biological Information
ImmunogenGST-tagged full-length human recombinant Microtubule-associated proteins 1A/1B light chain 3C (LC3C).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects LC3C.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAP1LC3C
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~17 kDa observed; 16.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3C in rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC2075-200UL 04054839550935