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ABS1633 Anti-IST1 Homolog

Anti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting.

Anti-IST1 Homolog MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: IST1, KIAA0174 UniProt Number: P53990
ABS1633
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1633
DescriptionAnti-IST1 Homolog
Alternate Names
  • hIST1
  • Putative MAPK-activating protein PM28
Background InformationIST1 homolog (UniProt: P53990; also known as hIST1, Putative MAPK-activating protein PM28) is encoded by the IST1 (also known as KIAA0174) gene (Gene ID: 9798) in human. IST1 is an ESCRT-III-like protein that is involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis. IST1 is also involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, it is involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. It is shown to regulate early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. Six isoforms of IST1 homolog have been described that are generated by alternative splicing.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected IST1 Homolog in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected IST1 Homolog in Western Blotting applications (Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62; Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).

Immunofluorescence Analysis: A representative lot detected IST1 Homolog in Immunofluorescence applications (Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).
Biological Information
ImmunogenA recombinant fragment corresponding to the first 189 amino acids from the N-terminal half of human IST1 homolog.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects IST1 homolog in human cells. It targets an epitope within the first 189 amino acids from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IST1
  • KIAA0174
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~50 kDa observed; 39.75 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IST1 Homolog in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1633 04054839361029