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ABN2294-100UL Anti-IQSEC3

Anti-IQSEC3, Cat. No. ABN2294, is a rabbit polyclonal antibody that detects IQSEC3 and is tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

ABN2294-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABN2294-100UL
DescriptionAnti-IQSEC3
Alternate Names
  • IQ motif and SEC7 domain-containing protein 3
  • Potential synaptic guanine nucleotide exchange factor for Arf
  • SynArfGEF-Po
  • BRAG3
Background InformationIQ motif and SEC7 domain-containing protein 3 (UniProt: Q76M68; also known as Potential synaptic guanine nucleotide exchange factor for Arf, SynArfGEF-Po) is encoded by the Iqsec3 (also known as Sag) gene (Gene ID: 404781) in rat. IQSEC3 along with IQSEC1 and IQSEC2 constitutes a family of brefeldin A-resistant ARF guanine nucleotide exchange factors (GEFs). It is a cytoplasmic protein that is expressed in the brain and is localized to dendrites and soma. Its expression is reported as early as E18 in hippocampal neurons. It is a key factor in controlling network activity in the hippocampus under both physiological and pathological conditions. It is shown to be exclusively localized to inhibitory synapses and it directly binds to gephyrin to promote inhibitory synapse formation in an ARF-GEF activity-dependent manner. IQSEC3 is reported to regulate GABAergic synapse maintenance in vivo and it links gephyrin-GABAA receptor complexes with ARF-dependent pathways to prevent aberrant, runaway excitation. Knockdown of Iqsec3 gene in the hippocampal dentate gyrus (DG) induces a significant reduction in GABAergic synapse density and causes a massive reduction of somatostatin expression in the DG hilus. IQ domain of IQSEC3 is localized to amino acids 311-340; the SEC7 domain is in amino acids 646-838; and the PH domain is in amino acids 852-985. (Ref.: Kim, S., et al. (2020). Cell Rep. 30(6); 1995-2005; Um, JW., et al. (2016). J. Biol. Chem. 291(19); 10119-10130).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in 10 mM Tris-HCl pH 7.5 with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IQSEC3, Cat. No. ABN2294, is a rabbit polyclonal antibody that detects IQSEC3 and is tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IQSEC3 in mouse brain tissue lysate.

Immunohistochemistry Applications: A representative lot detected IQSEC3 in Immunohistochemistry applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).

Immunocytochemistry Analysis: A representative lot detected IQSEC3 in Immunochemistry applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).

Immunoprecipitation Analysis: A representative lot detected IQSEC3 in Immunoprecipitation applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).

Western Blotting Analysis: A representative lot detected IQSEC3 in lysates from HEK293T cells transfected with Flag-IQSEC3, but not in untransfected cells. (Data Courtesy of Dr Jaewon Ko & Dr Seungjoon Kim at Daegu Gyeongbuk Institute of Science and Technology (DGIST)

Western Blotting Analysis: A representative lot detected IQSEC3 in Western Blotting applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the first 185 amino acids from rat IQ motif and SEC7 domain-containing protein 3 (IQSEC3).
EpitopeN-terminal
Concentration0.15 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects IQ motif and SEC7 domain-containing protein 3 (IQSEC3). It targets an epitope within the N-terminal domain.
IsotypeIgG
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteMouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Iqsec3
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~150 kDa observed; 129.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IQSEC3 in Rat brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2294-100UL 04065268041895