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ABE2882-100UL Anti-Histone H3 Antibody, K14M mutant

Anti-Histone H3, K14M mutant, Cat. No. ABE2882, is a highly specific rabbit polyclonal antibody that detects Histone H3 with K14M mutant has been tested for use in Dot Blot and Western Blotting.

ABE2882-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE2882-100UL
DescriptionAnti-Histone H3 Antibody, K14M mutant
Alternate Names
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistone H3.1 (UniProt: P68431; also known as Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l) is encoded by the HIST1H3A (also known as H3FA, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J) gene (Gene ID: 8350-8358, 8968) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. It has been reported that Histone H3.1 serves as the canonical histone, which is incorporated during DNA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription. Histone H 3.1 is a core component of nucleosome that is present only in mammals and is usually enriched in acetylation of Lysine 15 and demethylation of lysine 10 (HeK9Me2). It is expressed during S phase, then expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It s presence at the site of UV-induced DNA damage has also been reported. It has also been shown that H3.1/H4 tetramers do not split and remain intact during replication dependent deposition of H3.1 variant. This antibody specifically detects only the K14M mutated form of Histone H3 and does not react with the wild type. (Ref.: Stroud, H., et al., (2012). Proc. Natl. Acad. Sci. USA 109(14); 5370-5375).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Histone H3, K14M mutant, Cat. No. ABE2882, is a highly specific rabbit polyclonal antibody that detects Histone H3 with K14M mutant has been tested for use in Dot Blot and Western Blotting.
Key Applications
  • Dot Blot
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3, K14M mutant in whole cell extracts from 293T cells transfected with wildtype H3 or H3 K14M (Courtesy of Dr. Peter Lewis, University of Wisconsin).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids from the N-terminal region of Human Histone H3 with lysine 14 substituted with methionine.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Histone H3 with K14M substitution.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • HIST1H3C
  • HIST1H3D
  • HIST1H3E
  • HIST1H3F
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~19 kDa observed; 15.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated Anti-Histone H3, K14M mutant by Dot Blot using H3.1 peptide.



Dot Blot Analysis: 2.5 to 20 ng of this antibody detected Histone H3.1 peptide containing the K14M substitution.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2882-100UL 04061841030393