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ST1529 Anti-HTT Mouse mAb (3F1)

This Anti-HTT Mouse mAb (3F1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of HTT.

Anti-HTT Mouse mAb (3F1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HD, Anti-Huntingtin

ST1529
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1529-100UG 100 μg
Description
OverviewRecognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
Catalogue NumberST1529
Brand Family Calbiochem®
SynonymsAnti-HD, Anti-Huntingtin
Application Data
Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Clone3F1
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1529-100UG 04055977224214

Documentation

Anti-HTT Mouse mAb (3F1) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-HD, Anti-Huntingtin
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
BackgroundHuntington's disease is a gene-linked neurodegenerative disorder characterized by loss of striatal neurons. This disorder is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is ~13.7 kb and is expressed predominantly in adult and fetal brain, whereas the smaller transcript is ~10.3 kb and is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Clone3F1
IsotypeIgG2a
Specieshuman
Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review