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MABF3220-25UG Anti- HPV16 Antibody, clone H16.V5

Anti- HPV16, clone H16.V5, Cat. No. MABF3220, is mouse monoclonal antibody that detects HPV16 and is tested for use in and ELISA and Neutralizing Assay.

NewMABF3220-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF3220-25UG
DescriptionAnti- HPV16 Antibody, clone H16.V5
Alternate Names
  • Major capsid protein L1
  • HPV-16
Background InformationMajor capsid protein L1 (UniProt: P03101) is encoded by the L1 gene (Gene ID: 1489082) in Human papillomavirus type 16. The Major Capsid Protein L1 of Human papillomavirus is the primary structural protein responsible for the formation of the viral capsid, that encapsulates the genomic DNA together with Minor Capsid Protein L2. The viral capsid is composed of 360 copies of the L1 protein forming 72 pentamers, that assemble into the icosahedral capsid structure, while the L2 protein localizes along the inner surface of the virion and plays a role in capsid stabilization through interaction with the Major Capsid Protein L1. The L1 protein facilitates the attachment of the virus to the host cell by interacting with heparan sulfate proteoglycans on the cell surface, a critical step for infection. Recombinantly expressed Major Capsid Protein L1 can spontaneously assemble into Virus Like-Particles (VLPs) and were used for the development of neutralizing antibodies that show high degree of Human papillomavirus type-specificity. Based on their oncogenic potential, Human papillomaviruses (HPVs) can be classified as low-risk HPV types, (e.g.: HPV6 and HPV11, the most common serotypes) which do not cause cancer but can lead to skin and genital warts, and high-risk HPV types (e.g.: HPV 16, 18, 31, 33, 35, 45, 52, 58), that are responsible for HPV related cancers. Vaccines targeting the L1 protein, have been developed and are effective in preventing infection with the most oncogenic HPV types by inducing a robust antibody response. (Ref.: Hareza, D. A., (2022) Int. J. Mol. Sci., 23, 1818.; Christensen, N.D., et al. (1990). J Virol. 64:11, 5678-81; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Brown, M.J., et al. (2014). Clin Vaccine Immunol. 21:4, 587-93)
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives
Quality LevelMQ200
Applications
ApplicationAnti- HPV16, clone H16.V5, Cat. No. MABF3220, is mouse monoclonal antibody that detects HPV16 and is tested for use in and ELISA and Neutralizing Assay.
Key Applications
  • Neutralizing
  • ELISA
Application NotesTested Applications

ELISA Analysis: A representative lot of this antibody detected HPV16 protein in ELISA application (Data by Dr. J. Leitsch and with kind permission of ARTES Biotechnology GmbH, Germany. Recombinant HPV L1 antigens used were produced by ARTES Biotechnology GmbH).

Neutralizing: A representative lot detected HPV16 in Neutralizing application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Dias, D., et al. (2005). Clin Diagn Lab Immunol. 12(8):959-69; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

ELISA Analysis: A representative lot detected HPV16 in ELISA application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Panicker, G., et al. (2021). J Immunol Methods. 498:113136; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant baculovirus-induced HPV-16 L1 Virus Like-Particles (VLPs)
EpitopeUnknown
CloneH16.V5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone H16.V5 is a mouse monoclonal antibody that detects HPV16. It targets an epitope within the Major capsid protein L1. Might show some cross reactivity with HPV11.
IsotypeIgG2bκ
Species Reactivity
  • Virus
Species Reactivity NoteHuman Papillomavirus
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • L1
Purification MethodProtein G purified
UniProt Number
Molecular Weight56.28 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3220-25UG 04065272074025