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GF05 Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24)

Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-G-CSF

GF05
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.
Catalogue NumberGF05
Brand Family Calbiochem®
SynonymsAnti-G-CSF
References
ReferencesNicola, N.A. 1987. J. Cell Cloning 5, 1.
Metcalf, D., 1986. Blood 67, 257.
Nagata, S., et al. 1986. Nature 319, 415.
Nagata, S., et al. 1986 EMBO J. 5, 575.
Souza, L.M., et al. 1986 Science 232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlRecombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
Application CommentsFor paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified human G-CSF
ImmunogenHuman
Clone5.24
HostMouse
IsotypeIgG₂
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF05 0

Documentation

Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24) SDS

Title

Safety Data Sheet (SDS) 

Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24) Certificates of Analysis

TitleLot Number
GF05

References

Reference overview
Nicola, N.A. 1987. J. Cell Cloning 5, 1.
Metcalf, D., 1986. Blood 67, 257.
Nagata, S., et al. 1986. Nature 319, 415.
Nagata, S., et al. 1986 EMBO J. 5, 575.
Souza, L.M., et al. 1986 Science 232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-December-2008 RFH
SynonymsAnti-G-CSF
ApplicationImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.
BackgroundGranulocyte colony-stimulating factor (G-CSF) belongs to a family of hemopoietic growth factors controlling the survival, proliferation, differentiation and function of granulocytes, macrophages and their precursors. Other members of the family include interleukin-3 or multi-CSF, granulocyte-macrophage-CSF (GM-CSF) and macrophage-CSF (CSF-1). G-CSF was first described as a factor that induces differentiation in a murine myelomonocytic cell line WEHI-3BD and this observation was later confirmed using purified G-CSF. Murine and human G-CSF bind equally well to the receptors of either species and show complete biological cross-reactivity. Biochemically, human G-CSF is a hydrophobic glycoprotein of 19,000 molecular weight with a disperse range of isoelectric points (pI 5.5-6.1) due to glycosylation. The sequence of human cDNA clones isolated by two independent research groups differ only in the presence or absence of a sequence encoding 3 amino acid residues and shows a high degree of homology with murine G-CSF. The origin of the two mRNA species was latter identified as an alternative splice junction at the end of exon 2.
HostMouse
Immunogen speciesHuman
Immunogenpurified human G-CSF
Clone5.24
IsotypeIgG₂
Specieshuman
Positive controlRecombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesNicola, N.A. 1987. J. Cell Cloning 5, 1.
Metcalf, D., 1986. Blood 67, 257.
Nagata, S., et al. 1986. Nature 319, 415.
Nagata, S., et al. 1986 EMBO J. 5, 575.
Souza, L.M., et al. 1986 Science 232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today 5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem. 258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer 26, 647.