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DR1066 Anti-GTF2I Rabbit pAb

DR1066
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~130 kDa GTF2I protein.
Catalogue NumberDR1066
Brand Family Calbiochem®
SynonymsAnti-General Transcription Factor IIi
Application Data
Detection of human GTF2I by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with full-length human GTF2I. Antibody for IP: Anti-GTF2I Rabbit pAb (Cat. No. DR1066) (ratio of Ab to protein A beads 1:50-1:00). Antibody for IB: Anti-GTF2I Mouse pAb. Detection: chemiluminescence.
References
Product Information
FormulationUndiluted serum.
PreservativeNone
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsFor immunoprecipitation, the recommended ratio between antibody and protein A beads is 1:50 to 1:100; the amount of antibody is sample dependent. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human GTF2I (aa 1-976)
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1066 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-General Transcription Factor IIi
ApplicationImmunoprecipitation (see comments)
Application Data
Detection of human GTF2I by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with full-length human GTF2I. Antibody for IP: Anti-GTF2I Rabbit pAb (Cat. No. DR1066) (ratio of Ab to protein A beads 1:50-1:00). Antibody for IB: Anti-GTF2I Mouse pAb. Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa GTF2I protein.
BackgroundGTF2l is required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
HostRabbit
Immunogen speciesHuman
ImmunogenFull-length, human GTF2I (aa 1-976)
IsotypeIgG
Specieshuman
FormulationUndiluted serum.
PreservativeNone
CommentsFor immunoprecipitation, the recommended ratio between antibody and protein A beads is 1:50 to 1:100; the amount of antibody is sample dependent. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review