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AB1574-I-25UG Anti-GABA Transporter-3 Antibody, CT

Anti-GABA Transporter-3, CT, Cat. No. AB1574-I, is a rabbit polyclonal antibody that detects Sodium- and chloride-dependent GABA transporter 3 and is tested in Immunohistochemistry (Paraffin) and Western Blotting.

AB1574-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberAB1574-I-25UG
DescriptionAnti-GABA Transporter-3 Antibody, CT
Alternate Names
  • Sodium- and chloride-dependent GABA transporter 3
  • GAT-3
  • Solute carrier family 6 member 11
Background InformationSodium- and chloride-dependent GABA transporter 3 (UniProt: P31647; also known as GAT-3, Solute carrier family 6 member 11, GABA Transporter 3) is encoded by the Slc6a11 (also known as Gabt3, Gat-3, Gat-b) gene (Gene ID: 79213) in rat. GABA is reported to be the main inhibitory neurotransmitter in the mammalian brain, particularly in the basal ganglia network. Following its release from the presynaptic terminal, it is rapidly cleared from the extracellular space by GABA transporters (GATs) resulting it s the termination of inhibitory synaptic transmission. Thus far, four different GATs have been described in literature, GAT-1, GAT-2, GAT-3, and the Betain/GABA transporter type 1. GAT-3 is a multi-pass membrane protein that has one extracellular domain (aa 147-220), two cytoplasmic domains (aa 1-53 and 567-627), and twelve helical domains. It is shown to be highly expressed in the glomerular layer of the olfactory bulb, the inner nucleus of the retina, the thalamic paraventricular nucleus, and the globus pallidus. It terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. The GABA-transporting action of GATs is dependent on the Na+ gradient across the membrane. (Ref.: Jin XT., et al. (2011). Front. Syst. Neurosci. 5; 63).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-GABA Transporter-3, CT, Cat. No. AB1574-I, is a rabbit polyclonal antibody that detects Sodium- and chloride-dependent GABA transporter 3 and is tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal, cytoplasmic domain of rat GABA Transporter 3.
EpitopeC-terminus
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects GABA Transporter 3. It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slc6a11
  • Gabt3
  • Gat-3
  • Gat-b
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~59 Da observed; 69.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat Brain Microsomal Prep.

Western Blotting Analysis: 1 µg/mL of this antibody detected GABA Transporter-3 in Rat brain microsomal preparations.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB1574-I-25UG 04061842196326