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MABF1973 Anti-Etanercept Antibody, clone ETA63C8

Anti-Etanercept, clone ETA63C8, Cat. No. MABF1973, is a mouse monoclonal antibody that can bind to Etanercept and has been tested for use in ELISA and Neutralizing.

Anti-Etanercept Antibody, clone ETA63C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
MABF1973
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, NEUTMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1973
DescriptionAnti-Etanercept Antibody, clone ETA63C8
Alternate Names
  • Enbrel®
Background InformationEtanercept is a chimeric fusion protein consisting of the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. This Fc component contains the hinge, CH2 and CH3 regions, but not the CH1 region of IgG1. Etanercept binds to TNF-alpha and blocks its interactions with receptors. Hence, it is considered a TNF inhibitor that functions as a decoy receptor that binds to TNF. Although it mimics the inhibitory effects of naturally occurring soluble TNF receptors, the difference is that Etanercept has a greatly extended half-life in the bloodstream. Its speci fic activity is reported to be about 1.7 x 106 units/mg. Etanercept is shown to modulate biological responses produced by TNF, including expression of adhesion molecules responsible for leukocyte migration, serum levels of cytokines, and serum levels of matrix metalloproteinase-3. It is also shown to affect animal models of inflammation, including murine collagen-induced arthritis. Clone ETA63C8 can bind to Etanercept and block its activity.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Etanercept, clone ETA63C8, Cat. No. MABF1973, is a mouse monoclonal antibody that can bind to Etanercept and has been tested for use in ELISA and Neutralizing.
Key Applications
  • ELISA
  • Neutralizing
Application NotesNeutralizing Analysis: A representative lot neutralized Etanercept in Neutralizing applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).

ELISA Analysis: A representative lot detected Etanercept in ELISA applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).

ELISA Analysis: 4 µg/mL from a representative lot detected Etanercept in human serum with AS (ankylosing spondylitis) (Courtesy of Professor Ann Gils, Ph.D., KU Leuven Belgium).
Biological Information
ImmunogenChimeric fusion protein cotaining the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1.
CloneETA63C8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone ETA63C8 detects Etanercept in human samples. It can bind to Etanercept and block its activity.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Molecular Weight150 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1973 04054839362958