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AP1173 Anti-EPHA4 Mouse mAb (6H7)

Anti-EPHA4, mouse monoclonal, clone 6H7, recognizes the EPHA4 protein in NIH3T3 cells. It is validated for use in ELISA and Western blotting.

Anti-EPHA4 Mouse mAb (6H7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Ephrin Receptor A4

AP1173
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1173-100UG 100 μg
Description
OverviewRecognizes the EPHA4 protein in NIH3T3 cells.
Catalogue NumberAP1173
Brand Family Calbiochem®
SynonymsAnti-Ephrin Receptor A4
Application Data
Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlNIH3T3 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
Clone6H7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1173-100UG 04055977212716

Documentation

Anti-EPHA4 Mouse mAb (6H7) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Ephrin Receptor A4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa EPHA4 protein.
BackgroundThis protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
Clone6H7
IsotypeIgG₁
Specieshuman, mouse
Positive controlNIH3T3 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review