Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF2800-100UG Anti-EBNA1 Antibody, clone 1H4

Anti-EBNA1, clone 1H4, Cat. No. MABF2800, is a rat monoclonal antibody that detects EBNA-1 and is tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

MABF2800-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2800-100UG
DescriptionAnti-EBNA1 Antibody, clone 1H4
Alternate Names
  • Epstein-Barr nuclear antigen 1
  • EBNA-1
  • EBV nuclear antigen 1
Background InformationEpstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. Although the majority of virus infected individuals remain healthy, EBV has been implicated in the pathogenesis of African Burkitt s lymphoma and nasopharyngeal carcinoma. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-EBNA1, clone 1H4, Cat. No. MABF2800, is a rat monoclonal antibody that detects EBNA-1 and is tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected EBNA1 in Western Blotting applications (Grasser, F.A., et al. (1994). Blood. 84(11):3792-8; Humme, S., et al. (2003). Proc Natl Acad Sci USA. 100(19):10989-94; AlQarni, S., et al. (2018). Oncogene. 37(29):3998-4012).

Immunohistochemistry Analysis: A representative lot detected EBNA1 in Immunohistochemistry applications (Grasser, F.A., et al. (1994). Blood. 84(11):3792-8; Herrmann, K., et al. (2003). Breast Cancer Res. 5(1):R13-7; Chatterjee, B., et al. (2019). PLoS Pathog. 15(5):e1007748).

Immunoprecipitation Analysis: A representative lot detected EBNA1 in Immunoprecipitation applications (Schepers, A., et al. (2001). EMBO J. 20(16):4588-602).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenLambda-cro/lacZ-EBNAl-fusion protein s emulsified with complete Freund s adjuvant.
EpitopeUnknown
Clone1H4
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 1H4 is a rat monoclonal antibody that detects Epstein-Barr nuclear antigen 1.
IsotypeIgG2aκ
Species Reactivity
  • Virus
Species Reactivity NoteEpstein -Barr virus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • EBNA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed; 56.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EBNA-1 in Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2800-100UG 04065267327679

Documentation

Anti-EBNA1 Antibody, clone 1H4 SDS

Title

Safety Data Sheet (SDS) 

Anti-EBNA1 Antibody, clone 1H4 Certificates of Analysis

TitleLot Number
Anti-EBNA1, clone 1H4 - 4010116 4010116
Anti-EBNA1, clone 1H4 - 4035073 4035073
Anti-EBNA1, clone 1H4 - 4057519 4057519
Anti-EBNA1, clone 1H4 - 4092700 4092700
Anti-EBNA1, clone 1H4 - 4095089 4095089
Anti-EBNA1, clone 1H4 - 4173647 4173647
Anti-EBNA1, clone 1H4 - 4178330 4178330
Anti-EBNA1, clone 1H4 - 4182412 4182412
Anti-EBNA1, clone 1H4 - 4187023 4187023
Anti-EBNA1, clone 1H4 - 4190450 4190450
Show more