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MABS1180-25UG Anti-E3 ubiquitin-protein ligase UBR1 Antibody, clone 6H9.1

Anti-E3 ubiquitin-protein ligase UBR1, clone 6H9.1, Cat. No. MABS1180, is a mouse monoclonal that detects E3 ubiquitin-protein ligase UBR1 and has been tested for use in Immunohistochemistry (Paraffin) Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: UBR1 UniProt Number: Q8IWV7
MABS1180-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1180-25UG
DescriptionAnti-E3 ubiquitin-protein ligase UBR1 Antibody, clone 6H9.1
Alternate Names
  • EC: 2.3.2.27
  • N-recognin-1
  • RING-type E3 ubiquitin transferase UBR1
  • Ubiquitin-protein ligase E3-alpha-1
  • Ubiquitin-protein ligase E3-alpha-I
Background InformationE3 ubiquitin-protein ligase UBR1 (UniProt: Q8IWV7; also known as EC: 2.3.2.27, N-Recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I) is encoded by the UBR1 gene (Gene ID: 197131) in human. E3 ubiquitin-protein ligase UBR1 is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It may also be involved in pancreatic homeostasis. Two isoforms of UBR1 have been described that are produced by alternative splicing. UBR1 is broadly expressed protein with highest levels reported in skeletal muscle, kidneys, and pancreas. UBR1 has two zinc finger domains. The UBR-type domain is localized to amino acids 97 to 168 and it forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for arginine in first position and displays poor affinity for histidine. The second zinc finger domain, RING-type, atypical, is localized to amino acids 1098-1201. Mutations in UBR1 gene are causative factor in Johanson-Blizzard syndrome that is characterized by congenital exocrine pancreatic insufficiency, malformations such as nasal wing aplasia, and mental retardation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-E3 ubiquitin-protein ligase UBR1, clone 6H9.1, Cat. No. MABS1180, is a mouse monoclonal that detects E3 ubiquitin-protein ligase UBR1 and has been tested for use in Immunohistochemistry (Paraffin) Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected E3 ubiquitin-protein ligase UBR1 in human pancreas and human kidney tissue sections.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected E3 ubiquitin-protein ligase UBR1 in C6 cell lysate.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR1.
Clone6H9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6H9.1 detects E3 ubiquitin-protein ligase UBR1 in human and rat cells. It targets an epitope within 101 amino acids from the internal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBR1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 200.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UBR1 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1180-25UG 04054839441233