Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More
DR1095
Sigma-AldrichAnti-E2F2 Rabbit pAb
Anti-E2F2, rabbit polyclonal, recognizes the ~40-45 kDa E2F2 protein in HeLa cells and in mouse kidney. It is validated for use in Western blotting, and immunocytochemistry.
More>>Anti-E2F2, rabbit polyclonal, recognizes the ~40-45 kDa E2F2 protein in HeLa cells and in mouse kidney. It is validated for use in Western blotting, and immunocytochemistry. Less<<
Anti-E2F2 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~40-45 kDa E2F2 protein in HeLa cells and mouse kidney tissue.
Catalogue Number
DR1095
Brand Family
Calbiochem®
Synonyms
Anti-E2F Transcription Factor 2
Application Data
Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence. Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence. Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
Description
Purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa E2F2 protein.
Background
The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds specifically to retinoblastoma protein in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
Host
Rabbit
Immunogen species
Human
Immunogen
Full-length, human E2F2 (aa 1-437)
Isotype
IgG
Species
human, mouse
Positive control
Mouse kidney tissue, HeLa cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).