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DR1095 Anti-E2F2 Rabbit pAb

DR1095
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1095-100UG 100 μg
Description
OverviewRecognizes the ~40-45 kDa E2F2 protein in HeLa cells and mouse kidney tissue.
Catalogue NumberDR1095
Brand Family Calbiochem®
SynonymsAnti-E2F Transcription Factor 2
Application Data
Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlMouse kidney tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1:1200)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human E2F2 (aa 1-437)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
DR1095-100UG 04055977226140