Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABC451 Anti-Dihydropyrimidine dehydrogenase/DPYD Antibody

This Anti-Dihydropyrimidine dehydrogenase/DPYD Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Dihydropyrimidine dehydrogenase/DPYD.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DPYD UniProt Number: Q12882
ABC451
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC451
DescriptionAnti-Dihydropyrimidine dehydrogenase/DPYD Antibody
Alternate Names
  • Dihydropyrimidine dehydrogenase [NADP(+)]
  • DHPDHase
  • DPD
  • Dihydrothymine dehydrogenase
  • Dihydrouracil dehydrogenase
  • DPYD
Background InformationDPYD is an important enzyme involved in pyrimidine base degradation and catabolism. DPYD catalyzes the reduction of uracil and thymine and also is a critical enzyme in the degradation of the chemotherapeutic drug 5-fluoruracil. DPYD is expressed in most cells and has highest activity in the liver and monocytes. Mutations in DPYD give rise to a range of symptoms and syndromes in patients ranging from no symptoms to highly convulsive and mental retardation caused by excessive amounts of uracil or thymine present in the blood. Because of its ability to degrade 5-fluorouracil, DPYD is studied as a cancer marker and a measure of therapeutic effects in many cancers. Recent research also shows that DPYD levels in the liver are regulated via microRNA interactions and an offer a new opportunity for modulation of this critical enzyme in health and disease.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Dihydropyrimidine dehydrogenase/DPYD Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Dihydropyrimidine dehydrogenase/DPYD.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected Dihydropyrimidine dehydrogenase/DPYD in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dihydropyrimidine dehydrogenase/DPYD in human liver tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Dihydropyrimidine dehydrogenase/DPYD near the N-terminus.
EpitopeNear N-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Primate, Bovine, Porcine, Horse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DPYD
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~110 kDa observed. Uncharacterized band at ~47 kDa may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Dihydropyrimidine dehydrogenase/DPYD in 10 µg of human small intestine tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC451 04055977109191