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AP1174 Anti-DLC1 Rabbit pAb

AP1174
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
OverviewRecognizes the ~200-250 kDa DLC1 protein.
Catalogue NumberAP1174
Brand Family Calbiochem®
SynonymsAnti-STARD 12, Anti-Deleted in Liver Cancer 1
Application Data
Detection of human DLC1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with DLC1. Antibody for IP: Anti-DLC1 Rabbit pAb (Cat. No. AP1174) (ratio of Ab: protein A beads is 1:50-1:100). Antibody for IB: Anti-DLC1 Mouse pAb. Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationUndiluted serum.
PreservativeNone
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsFor immunoprecipitation, the ratio of antibody to protein A beads should be 1:50-1:100, but the dilution of antibody needs to be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human DLC1 (aa 1-1528)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1174 0

Documentation

Anti-DLC1 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-STARD 12, Anti-Deleted in Liver Cancer 1
ApplicationImmunoprecipitation (see comments)
Application Data
Detection of human DLC1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with DLC1. Antibody for IP: Anti-DLC1 Rabbit pAb (Cat. No. AP1174) (ratio of Ab: protein A beads is 1:50-1:100). Antibody for IB: Anti-DLC1 Mouse pAb. Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~200-250 kDa DLC1 protein.
BackgroundDLC1 is a protein which in humans is encoded by the DLC1 gene. This gene is deleted in the primary tumor of hepatocellular carcinoma. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal and breast cancers.
HostRabbit
Immunogen speciesHuman
ImmunogenFull-length, human DLC1 (aa 1-1528)
IsotypeIgG
Specieshuman
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsFor immunoprecipitation, the ratio of antibody to protein A beads should be 1:50-1:100, but the dilution of antibody needs to be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review