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ABS222 Anti-DEPTOR Antibody

Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.

Anti-DEPTOR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: DEPTOR, DEPDC6 UniProt Number: Q8TB45
ABS222
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS222
Replaces09-463
DescriptionAnti-DEPTOR Antibody
Alternate Names
  • DEP domain-containing mTOR-interacting protein
  • DEP domain-containing protein 6
Background InformationDEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • HEK293 cell lyaste
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.
EpitopeDEP domain-containing mTOR-interacting protein DEPTOR.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human.
Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DEPTOR
  • DEPDC6
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.

SUBUNIT STRUCTUREInteracts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.
Molecular Weight~46 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS222 04053252632846