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AP1046 Anti-CtIP Rabbit pAb

Anti-CtIP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1046
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~125 kDa CtIP protein in MCF7 cells.
Catalogue NumberAP1046
Brand Family Calbiochem®
Application Data
Lane 1: Detection of CtIP by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF7 cells. Primary antibody: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (1 µg/ml). Detection: chemiluminescence. Lane 2/3: Detection of CtIP by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from MCF7 cells. Antibodies for IP: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (3 µg/mg protein) (lane 2) and Rabbit IgG (3 µg/mg protein) (lane 3). Immunoblotting conditions: same as above.
References
ReferencesChen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
Germani, A., et al. 2003. Oncogene 22, 8845.
Li, S., et al. 2000. Nature 406, 210.
Wong, A.K., et al. 1998. Oncogene 17, 2279.
Product Information
FormLiquid
FormulationIn Tris citrate/phosphate buffer, pH 7-8.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (3 µg/mg protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human CtIP
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1046 0

Documentation

Anti-CtIP Rabbit pAb Certificates of Analysis

TitleLot Number
AP1046

References

Reference overview
Chen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
Germani, A., et al. 2003. Oncogene 22, 8845.
Li, S., et al. 2000. Nature 406, 210.
Wong, A.K., et al. 1998. Oncogene 17, 2279.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Immunoprecipitation (3 µg/mg protein)
Application Data
Lane 1: Detection of CtIP by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF7 cells. Primary antibody: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (1 µg/ml). Detection: chemiluminescence. Lane 2/3: Detection of CtIP by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from MCF7 cells. Antibodies for IP: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (3 µg/mg protein) (lane 2) and Rabbit IgG (3 µg/mg protein) (lane 3). Immunoblotting conditions: same as above.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~125 kDa CtIP protein in MCF7 cells.
BackgroundCtBP interacting protein (CtIP; Accession No. NP_002885), originally identified as a binding partner of the transcriptional suppressor CtBP, was subsequently shown by two-hybrid screening to interact with BRCA1 BRCT domains. CtIP is a phosphorylation-dependent binding partner of the BRCA1 BRCT domains. The cell cycle-regulated BRCA1/CtIP complex is required for the G2/M transition and Chk1 activation following DNA damage. CtIP interacts with a group of tumor suppressor proteins including Rb (retinoblastoma protein), BRCA1, Ikaros, and CtBP, which regulate cell cycle progression through transcriptional repression as well as chromatin remodeling. CtIP associates with SIAH-1 both in vitro and in vivo leading to CtIP degradation by the ubiquitin-proteasome pathway. CtIP(-/-) embryos die at embryonic day 4 with blastocysts arrested in G1, in an Rb dependent manner. CtIP(+/-) heterozygotes are characterized by the development of multiple types of tumors, predominantly, large lymphomas. CtIP becomes hyperphosphorylated and dissociated from BRCA1 upon exposure to ionizing radiation. This phosphorylation event requires the protein kinase, ATM, that is mutated in the disease ataxia telangiectasia. ATM phosphorylates CtIP at Ser664 and Ser745. ATM modulates BRCA1-mediated regulation of the DNA damage-response GADD45 gene through CtIP, thus providing a potential link between ATM deficiency and breast cancer.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human CtIP
IsotypeIgG
Specieshuman
Positive controlMCF7 cells
FormLiquid
FormulationIn Tris citrate/phosphate buffer, pH 7-8.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
Germani, A., et al. 2003. Oncogene 22, 8845.
Li, S., et al. 2000. Nature 406, 210.
Wong, A.K., et al. 1998. Oncogene 17, 2279.