Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB3678-I-100UL Anti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1

This mouse monoclonal Anti-Chloramphenicol Acetyltransferase, clone CAT-1, Cat. No. MAB3678-I is tested for use in Immunocytochemistry and Western Blotting and Immunofluorescence, for the detection of Chloramphenicol acetyltransferase.

MAB3678-I-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB3678-I-100UL
DescriptionAnti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1
Alternate Names
  • EC:2.3.1.28
  • CAT
Background InformationChloramphenicol acetyltransferase (UniProt: P62577; also known as EC:2.3.1.28, CAT) is encoded by the Cat gene (Gene ID: 59683728) in E. coli. Chloramphenicol acetyltransferase is an enzyme that catalyzes the acetyl-S-CoA-dependent acetylation of chloramphenicol at the 3-hydroxyl group leading to the production of 3-acetoxy chloramphenicol that does not bind to bacterial ribosomes and does not inhibit peptidyltransferase activity. It displays high stability in cells and has a half-life of about 48 hours. A highly reactive histidine residue has been implicated in the catalytic mechanism. CAT is shown to be an effector of chloramphenicol resistance in bacteria. Its synthesis is shown to be constitutive in E. coli and other Gram-negative bacteria that harbor plasmids bearing the structural gene for the enzyme. However, in Gram-positive bacteria such as staphylococci and streptococci it is synthesized only in the presence of chloramphenicol and related compounds. (Ref.:Shaw WH. (1983). CRC Crit. Rev. Biochem. 14(1); 1-46).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis mouse monoclonal Anti-Chloramphenicol Acetyltransferase, clone CAT-1, Cat. No. MAB3678-I is tested for use in Immunocytochemistry and Western Blotting and Immunofluorescence, for the detection of Chloramphenicol acetyltransferase.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected Chloramphenicol acetyltransferase in Immunofluorescence applications (Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

Western Blotting Analysis: A representative lot detected Chloramphenicol acetyltransferase in Western Blotting applications (Smith, M.C., et al. (2011). Antiviral Res.;91(3):259-66; Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant chloramphenicol acetyl transferase.
EpitopeUnknown
CloneCAT-1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CAT-1 is a mouse monoclonal antibody that detects Chloramphenicol acetyltransferase.
IsotypeIgG1
Species Reactivity
  • E. coli
Species Reactivity NoteE. Coli. Predicted to react with all species.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cat
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.66 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HEK293 cells transfected with gWIZ CAT plasmid.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Chloramphenicol Acetyltransferase in HEK293 cells transfected with gWIZ CAT plasmid.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3678-I-100UL 04065266791433