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219361 Anti-Cathepsin D Rabbit pAb

Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis.

Anti-Cathepsin D Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
219361
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
219361-1ML Plastic ampoule 1 ml
Description
OverviewRecognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.
Catalogue Number219361
Brand Family Calbiochem®
Application Data
Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHuman liver tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoelectrophoresis
Application NotesELISA (≥1:1000)
Immunoelectrophoresis (see comments)
Application CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified human liver cathepsin D
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
219361-1ML 04055977202199

Documentation

Anti-Cathepsin D Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Cathepsin D Rabbit pAb Certificates of Analysis

TitleLot Number
219361

Brochure

Title
Cathepsins and Related Products Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
ApplicationELISA (≥1:1000)
Immunoelectrophoresis (see comments)
Application Data
Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
HostRabbit
Immunogen speciesHuman
Immunogenpurified human liver cathepsin D
IsotypeIgG
Specieshuman
Positive controlHuman liver tissue
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling