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AM46 Anti-Caspase-8 (Ab-3) Mouse mAb (1-3)

Anti-Caspase-8, mouse mAb, clone 1-3, recognizes ~55-58 kDa latent form in non-apoptotic Jurkat cells & ~18 kDa active form in apoptotic Jurkat & HeLa cells. It is validated for Western blotting.

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5

AM46
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~55-58 kDa latent form of caspase-8 in non-apoptotic Jurkat cells and with ~18 kDa active form in apoptotic Jurkat or HeLa cells. Additional proteins at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines.
Catalogue NumberAM46
Brand Family Calbiochem®
SynonymsAnti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5
References
ReferencesSmyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Juo, P., et al. 1998. Curr. Biol. 8, 1001.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Muzio, M., et al. 1996. Cell 85, 817.
Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
Positive controlHeLa or Jurkat cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Not Frozen Sections
Not Immunofluorescence
Not Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
Application CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman recombinant caspase-8
ImmunogenHuman
Clone1-3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM46 0

Documentation

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) SDS

Title

Safety Data Sheet (SDS) 

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) Certificates of Analysis

TitleLot Number
AM46

References

Reference overview
Smyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Juo, P., et al. 1998. Curr. Biol. 8, 1001.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Muzio, M., et al. 1996. Cell 85, 817.
Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.

Citations

Title
  • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-August-2007 RFH
    SynonymsAnti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5
    ApplicationImmunoblotting (1 µg/ml)
    Frozen Sections (not recommended)
    Immunofluorescence (not recommended)
    Immunoprecipitation (not recommended)
    Paraffin Sections (not recommended)
    DescriptionPurified mouse monoclonal antibody. Recognizes the ~56-58 kDa latent and the ~18 kDa active form of caspase-8.
    BackgroundCaspase-8 (ICE-LAP5, MACH, FLICE, CAP4) is the most proximal of the members of the apoptotic caspase cascade. Caspase-8 is directly involved in apoptosis mediated through either Fas or the TNF receptor and forms one of the key components of the transducing DISC, the CAP4 protein. Cell lines that are deficient in caspase-8 are resistant to Fas mediated apoptosis. Nine different isoforms of caspase 8 have been identified but the "a" and "b" isoforms show the highest expression at the level of protein in multiple tissues. Caspase-8 has a pH optimum of 6.8 and has a kcat/Km of 8.7 X 105 M-1 s-1. The pro-form of caspase-8 has a molecular weight of approximately 55 kDa and undergoes several processing steps to generate the active heterodimer of p18 and p10/12. In addition to activation via Fas, oligomerization of caspase-8 at the cell membrane and interaction with Granzyme B and caspase 10 will induce auto-processing and activation of the apoptotic pathway. The mouse caspase-8 gene has also been identified and is expressed in the adult as well as at high levels in the day 9.5 and day 17.5 embryo suggesting a role in development.
    HostMouse
    Immunogen speciesHuman
    Immunogenhuman recombinant caspase-8
    Clone1-3
    IsotypeIgG₁
    Specieshuman
    Positive controlHeLa or Jurkat cells
    Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesSmyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
    Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
    Juo, P., et al. 1998. Curr. Biol. 8, 1001.
    Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
    Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
    Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
    Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
    Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
    Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
    Muzio, M., et al. 1996. Cell 85, 817.
    Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
    Citation
  • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
    		•