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AB356492 Anti-Cas9 (Chicken Polyclonal)

Anti-Cas9 (Chicken Polyclonal), Cat. No. AB356492, is a highly specific chicken polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.

Anti-Cas9 (Chicken Polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas9 UniProt Number: J7RUA5
AB356492
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
BactIF, WBChUnpurifiedPolyclonal Antibody
Description
Catalogue NumberAB356492
DescriptionAnti-Cas9 (Chicken Polyclonal)
Alternate Names
  • SaCas9
  • CRISPR-associated endonuclease Cas9
Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.
References
Product Information
FormatUnpurified
PresentationChicken polyclonal antibody in PBS with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cas9 (Chicken Polyclonal), Cat. No. AB356492, is a highly specific chicken polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 251 amino acids from the C-terminal end of CRISPR-associated endonuclease Cas9 from Staphylococcus aureus.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostChicken
SpecificityThis chicken polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. This antibody does not react with Cas9 from Streptococcus pyogenes.
IsotypeIgY
Species Reactivity
  • Bacteria
Species Reactivity NoteStaphylococcus aureus.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cas9
Purification MethodUnpurified
UniProt Number
Molecular Weight~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cas9 in 40 µg of lysate from HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB356492 04054839355516