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PC711 Anti-CHIP Rabbit pAb

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.

Anti-CHIP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Carboxyl Terminus of Hcs70-Interacting Protein

PC711
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Dr, Ht, H, Mk, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC711-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
Catalogue NumberPC711
Brand Family Calbiochem®
SynonymsAnti-Carboxyl Terminus of Hcs70-Interacting Protein
References
ReferencesXu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
Demand, J., et al. 2001. Curr. Biol. 11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlCOS-7 cells
Preservative≤0.1% Sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
Application CommentsMay also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of full-length human CHIP fused to a His•Tag® sequence
HostRabbit
IsotypeIgG
Species Reactivity
  • Drosophila
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC711-100UL 04055977207897

Documentation

Anti-CHIP Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-CHIP Rabbit pAb Certificates of Analysis

TitleLot Number
PC711

References

Reference overview
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
Demand, J., et al. 2001. Curr. Biol. 11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2018 JSW
SynonymsAnti-Carboxyl Terminus of Hcs70-Interacting Protein
ApplicationImmunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.
BackgroundCHIP is a U-box dependent E3 ubiquitin ligase and a co-chaperone protein that interacts with Hsc70 and Hsp90 regulating protein triage decisions between folding and degradation. This occurs through CHIP remodeling the complexes to favor substrate degradation thus providing a molecular link between the chaperone and proteosome machinery. CHIP has been reported to be involved in the degradation of c-ErbB2/c-Neu, the glucocorticoid receptor, and the cystic fibrosis transmembrane-conductance regulator. CHIP expression shortens the half-life of both the nascent and mature ErbB2 protein.
HostRabbit
Immunogena recombinant protein consisting of full-length human CHIP fused to a His•Tag® sequence
IsotypeIgG
SpeciesDrosophila, hamster, human, monkey, mouse, rat
Positive controlCOS-7 cells
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% Sodium azide
CommentsMay also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesXu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
Demand, J., et al. 2001. Curr. Biol. 11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.