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MABE1114 Anti-CENP-C Antibody, clone AL61A

Anti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.

Anti-CENP-C Antibody, clone AL61A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: CENPC, CENPC1, ICEN7 UniProt Number: Q03188
MABE1114
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1114
DescriptionAnti-CENP-C Antibody, clone AL61A
Alternate Names
  • Centromere protein C
  • CENP-C
  • CENP-C 1
  • Centromere autoantigen C
  • Centromere protein C 1
  • Interphase centromere complex protein 7
Background InformationCentromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to casue mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunoprecipitated CENP-C from HeLa nuclear extract (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant detected nuclear CENP-C puncta by fluorescent immunocytochemistry staining of permeabilized HeLa and Jurkat cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunostained CENP-C-transfected, but not ZBTB32-transfected, HEK293T cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Biological Information
ImmunogenGST-tagged recombinant human CENP-C N-terminal fragment.
EpitopeN-terminal.
CloneAL61A
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms of human CENP-C reported by UniProt (Q03188).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CENPC
  • CENPC1
  • ICEN7
Purification MethodProtein G Purified
UniProt Number
Molecular Weight106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected CENP-C in Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1114 04055977333299