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MABF293 Anti-CD46 Antibody, clone 8A9.1

This Anti-CD46 Antibody, clone 8A9.1 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of CD46.

Anti-CD46 Antibody, clone 8A9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD46, MCP, MIC10 UniProt Number: P15529
MABF293
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF293
DescriptionAnti-CD46 Antibody, clone 8A9.1
Alternate Names
  • Membrane cofactor protein
  • TLX
  • Trophoblast leukocyte common antigen
  • CD46
  • CD46
Background InformationMembrane cofactor protein (UniProt P15529; also known as Antigen identified by monoclonal antibody TRA-2-10, CD46 antigen, Complement membrane cofactor protein, Measles virus receptor membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, Trophoblast-lymphocyte cross-reactive antigen) is encoded by the CD46 (also known as MCP, TLX, AHUS2, MIC10, TRA2.10) gene (Gene ID 4179) in human. Signal 1-34, Membrane cofactor protein (MCP) or CD46 is a widely expressed complement regulator that inhibits all three pathways of complement activation, classical (CP), alternative (AP), and lectin (LP). It binds to both C3b (AP) and C4b (CP) and acts as a cofactor for their inactivation by the C3b/C4b inactivator enzyme factor I. CD46 is initially produced with an N-terminal signal sequence (a.a. 1-34), which is post-translationally removed to yield the type 1 membrane bound CD46 consisting of a large extracellular (a.a. 35-343) portion, followed by a single transmembrane α-helical region (a.a. 344-366) and a cytoplasmic tail (a.a. 367-392) with several variant structures. The extracellular portion is composed of four extracellular Complement Control Protein (CCP) domains carrying the cofactor activity, a collar STP-rich region, and a short region of unknown function (U). CD46 also acts as a receptor for certain pathogens such as the measles virus (Edmonston strain), human herpesvirus 6, and Neisseria gonorrhoeae. CD46 is also implicated to have a role in human egg fertilization. Several mutations in the CCPs and the transmembrane region of CD46 are linked to atypical hemolytic uremic syndrome (aHUS).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD46 Antibody, clone 8A9.1 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of CD46.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected CD46 in HeLa and A431 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD46 in 1X10E6 HeLa cells.
Biological Information
ImmunogenmIg Fc-tagged recombinant protein corresponding to the extracellular domain of human CD46.
EpitopeExtracellular domain
Clone8A9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with isoforms A (no del, ABC1) and isoform B (del 13, ABC2). Reactivity toward other spliced isoforms (Uniprot P15529-3 through P15529-16) has not been determined.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD46
  • MCP
  • MIC10
Purification MethodProtein G Purified
UniProt Number
Molecular Weight43.7 kDa (isoforms A; no del ABC1) and 44.2 kDa (isoforms B; del 13 ABC2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human CD46 extracellular domain mIg Fc fusion protein.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CD46 in 0.25 µg of human CD46 extracellular domain mIg Fc fusion protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF293 04055977294330