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CBL496F Anti-CD34 Class II Antibody, clone QBEND/10, FITC conjugated

CBL496F
100 assays  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MkFCMFITCMonoclonal Antibody
Description
Catalogue NumberCBL496F
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CD34 Class II Antibody, clone QBEND/10, FITC conjugated
Product Information
FormatFITC
PresentationThe conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD34 Antibody (Class II), clone QBEND/10, FITC conjugated is validated for use in FC for the detection of CD34 Class II.
Key Applications
  • Flow Cytometry
Application NotesIdentification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman endothelial vesicles
CloneQBEND/10
HostMouse
SpecificityThe antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

FUSION PARTNER: NSO myeloma cell line
IsotypeIgG1
Species Reactivity
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
Gene Symbol
  • CD34
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
Product Usage Statements
Availability by Geography
  • This product is not available for sale in the United States.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
Packaging Information
Material Size100 assays
Global Trade Item Number
Catalogue Number GTIN
CBL496F 04053252369698