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MABF2031 Anti-CD1b Antibody, clone BCD1b3.1

Anti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function.

Anti-CD1b Antibody, clone BCD1b3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD1B UniProt Number: P29016
MABF2031
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Gp, HFC, IHC, Inhibition, Neutralization AssayMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2031
DescriptionAnti-CD1b Antibody, clone BCD1b3.1
Alternate Names
  • T-cell surface glycoprotein CD1b
Background InformationT-cell surface glycoprotein CD1b (UniProt: P29016; also known as CD1b) is encoded by the CD1B gene (Gene ID: 910) in human. CD1b is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. The most prominent known function of CD1b molecules is to associate with lipids from pathogens in order to stimulate T cells and induce host defense mechanisms. CD1b is expressed on cortical thymocytes, certain T-cell leukemia, and other tissues. Two isoforms of CD1b are reported that are produced by alternative splicing. CD1b has an extracellular domain (aa 18-3-3), a helical domain (aa 304-324), and a cytoplasmic tail (aa 329-332). The newly synthesized CD1b molecules follow an intracellular transport pathway that leads it to the cell surface from endoplasmic reticulum and subsequently to internalization from the plasma membrane into the endocytic pathway. After internalization, CD1b molecules are sorted for transport to lysosomes in the sorting endosome. (Ref.: Briken, V., et al. (2002). EMBO J. 21(4); 825-834).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Inhibition
  • Neutralization Assay
Application NotesImmunohistochemistry Analysis: A representative lot detected CD1b in Immunohistochemistry applications (Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).

Flow Cytometry Analysis: A representative lot detected CD1b in Flow Cytometry applications (Hiromatsu, K., et. al. (2002). Immunology. 106(2):159-72; Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88; Sieling, P.A., et. al. (2005). J Immunol. 174(5):2637-44; Facciotti, F., et. al. (2011). Proc Natl Acad Sci USA. 108(34):14228-33).

Immunoprecipitation Analysis: A representative lot detected CD1b in Immunoprecipitation applications (Briken, V., et. al. (2002). EMBO J. 21(4):825-34; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).

Neutralizing Analysis: A representative lot detected CD1b in Neutralizing applications (Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46).

Inhibition Analysis: A representative lot detected CD1b in Inhibition applications (Behar, S.M., et. al. (1995). J Exp Med. 182(6):2007-18).
Biological Information
ImmunogenIsolated human CD1+ monocytes.
CloneBCD1b3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone BCD1b3.1 detects T-cell surface glycoprotein CD1b in human and guinea pig.
IsotypeIgG1κ
Species Reactivity
  • Guinea Pig
  • Human
Species Reactivity NoteGuinea Pig, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD1B
Purification MethodProtein G purified
UniProt Number
Molecular Weight36.94 kda calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in MOLT4 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD1b in one million MOLT4 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2031 04054839352614