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MABN1547 Anti-CASK Antibody, clone 2G5.1

This Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.

Anti-CASK Antibody, clone 2G5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: CASK, LIN2 UniProt Number: O14936
MABN1547
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1547
ReplacesMAB5230
DescriptionAnti-CASK Antibody, clone 2G5.1
Alternate Names
  • Peripheral plasma membrane protein CASK
  • hCASK
  • Calcium/calmodulin-dependent serine protein kinase
  • Protein lin-2 homolog
  • CASK
Background InformationPeripheral plasma membrane protein CASK (EC 2.7.11.1; UniProt O14936; also known as Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, hCASK, Protein lin-2 homolog, Trinucleotide repeat containing 8) is encoded by the CASK (also known as CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8) gene (Gene ID 8573) in human. CASK is a membrane-associated guanylate kinase (MAGUK) family member involved in the regulation of cytoskeletal remodeling and cell migration. It consists of a Ca2+-calmodulin kinase, a PDZ, an SH3, and a guanylate kinase domain. CASK localizes at cell–cell adhesion sites, including synaptic junctions, epithelial junctions, and tight junctions, where it is reported to associate with other cell adhesion molecules, such as junctional adhesion molecule (JAM), cadherins, catenins, and syndecan-2. CASK and syndecan-2 expressions are upregulated in colorectal cancer and their upregulation significantly correlates with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CASK in human kidney cortex tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to an internal fragment within the C-terminal half of human CASK.
EpitopeInternal (C-terminal half)
Clone2G5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteRat, Human. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CASK
  • LIN2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~115 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CASK in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1547 04055977182811