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MAB3222 Anti-BrdU Antibody, clone IIB5

MAB3222
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AFC, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3222
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-BrdU Antibody, clone IIB5
Alternate Names
  • BrdU
Background InformationThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • After incorporation of BrdU, all DNA containing species
PresentationProtein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:

http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.

Flow Cytometry: 1:100-1:200; acid methanol treatment required.

Optimal working dilutions must be determined by the end user.

In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
Biological Information
ImmunogenA fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
CloneIIB5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
IsotypeIgG1
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3222 04053252666414

Documentation

Anti-BrdU Antibody, clone IIB5 SDS

Title

Safety Data Sheet (SDS) 

Anti-BrdU Antibody, clone IIB5 Certificates of Analysis

TitleLot Number
Anti-Bromodeoxyuridine, clone IIB5 3060051
Anti-Bromodeoxyuridine, clone IIB5 - 2382096 2382096
Anti-Bromodeoxyuridine, clone IIB5 - 2465131 2465131
Anti-Bromodeoxyuridine, clone IIB5 - 1959566 1959566
Anti-Bromodeoxyuridine, clone IIB5 - 2028282 2028282
Anti-Bromodeoxyuridine, clone IIB5 - 2154057 2154057
Anti-Bromodeoxyuridine, clone IIB5 - 2295630 2295630
Anti-Bromodeoxyuridine, clone IIB5 - 2991040 2991040
Anti-Bromodeoxyuridine, clone IIB5 - 3170852 3170852
Anti-Bromodeoxyuridine, clone IIB5 - 3230505 3230505

References

Reference overviewApplicationPub Med ID
Expression of 1N3R-Tau isoform inhibits cell proliferation by inducing S phase arrest in N2a cells.
Li, L; Xu, ZP; Liu, GP; Xu, C; Wang, ZH; Li, XG; Liu, EJ; Zeng, J; Chai, DM; Yao, WL; Wang, JZ
PloS one  10  e0119865  2015

Show Abstract
Immunocytochemistry25822823 25822823
Activity-dependent modulation of odorant receptor gene expression in the mouse olfactory epithelium.
Zhao, S; Tian, H; Ma, L; Yuan, Y; Yu, CR; Ma, M
PloS one  8  e69862  2013

Show Abstract
23922828 23922828
Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via TGF-β-TAK1-dependent mechanism.
Shang, Y; Doan, CN; Arnold, TD; Lee, S; Tang, AA; Reichardt, LF; Huang, EJ
PLoS biology  11  e1001527  2013

Show Abstract
23565059 23565059
The colony-stimulating factor-1 (CSF-1) receptor sustains ERK1/2 activation and proliferation in breast cancer cell lines.
Morandi, A; Barbetti, V; Riverso, M; Dello Sbarba, P; Rovida, E
PloS one  6  e27450  2011

Show Abstract Full Text Article
22096574 22096574
Interactions of Wnt/beta-catenin signaling and sonic hedgehog regulate the neurogenesis of ventral midbrain dopamine neurons.
Tang, M; Villaescusa, JC; Luo, SX; Guitarte, C; Lei, S; Miyamoto, Y; Taketo, MM; Arenas, E; Huang, EJ
The Journal of neuroscience : the official journal of the Society for Neuroscience  30  9280-91  2010

Show Abstract
Immunohistochemistry20610763 20610763

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies