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OP60 Anti-Bcl-2 (Ab-1) Mouse mAb (100)

Anti-Bcl-2 (Ab-1) Mouse mAb (100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2α

OP60
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, tonsil, and lymph node tissue.
Catalogue NumberOP60
Brand Family Calbiochem®
SynonymsAnti-Bcl-2α
References
ReferencesKozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlMCF7 cells or tonsil or lymph node tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Pezzella, F., et al. 1990. Am. J. Pathol. 137, 225.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, see comments)
Application CommentsGenerally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogena synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
ImmunogenHuman
Clone100
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP60 0

Documentation

Anti-Bcl-2 (Ab-1) Mouse mAb (100) SDS

Title

Safety Data Sheet (SDS) 

Anti-Bcl-2 (Ab-1) Mouse mAb (100) Certificates of Analysis

TitleLot Number
OP60

References

Reference overview
Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
SynonymsAnti-Bcl-2α
ApplicationFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells (see application references). Recognizes the ~24-26 kDa bcl-2 protein.
BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death". It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
Clone100
IsotypeIgG₁
Specieshuman, not mouse
Positive controlMCF7 cells or tonsil or lymph node tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsGenerally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesKozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.
Application referencesOriginal Clone Pezzella, F., et al. 1990. Am. J. Pathol. 137, 225.